Tachibana M, Morioka H
Department of Otolaryngology, Meiji College of Oriental Medicine, Kyoto, Japan.
Hear Res. 1992 Sep;62(1):11-5. doi: 10.1016/0378-5955(92)90198-v.
Localization of glucuronic acid-containing glycosaminoglycans in the gerbil utricle was examined, using a hyaluronidase-gold labeling technique with special emphasis on the otoconia. Otoconia and the gelatinous layer of the otoconial membrane were strongly labeled by hyaluronidase-gold. The secretory granules in supporting cells were also strongly labeled, suggesting that the organic matrix of otoconia is secreted from the supporting cells. Otoconia seem to lose labeling while they degenerated. The degenerating otoconia were observed to be absorbed into dark cells. Glucuronic acid-containing glycosaminoglycans occur in otoconia. These glycosaminoglycans may play a crucial role in the formation and degeneration of otoconia.
利用透明质酸酶-金标记技术，特别着重于耳石，对沙鼠椭圆囊内含有葡萄糖醛酸的糖胺聚糖进行了定位研究。耳石及耳石膜的凝胶层被透明质酸酶-金强烈标记。支持细胞中的分泌颗粒也被强烈标记，这表明耳石的有机基质是由支持细胞分泌的。耳石在退化时似乎会失去标记。观察到退化的耳石被暗细胞吸收。耳石中存在含有葡萄糖醛酸的糖胺聚糖。这些糖胺聚糖可能在耳石的形成和退化过程中起关键作用。
