DELEY J, FRIEDMAN S
J Bacteriol. 1965 May;89(5):1306-9. doi: 10.1128/JB.89.5.1306-1309.1965.
De Ley, J. (State University, Ghent, Belgium), and S. Friedman. Similarity of Xanthomonas and Pseudomonas deoxyribonucleic acid. J. Bacteriol. 89:1306-1309. 1965.-Pronounced deoxyribonucleic acid (DNA) hybridization was detected with the DNA-agar method within the group of Xanthomonas and between Xanthomonas and Pseudomonas. Sheared, denatured C(14)-DNA from X. pelargonii hybridized 80 to 100% with high molecular weight, denatured agar-bound DNA from eight different Xanthomonas nomenspecies. The near identity of the DNA in these nine strains favors their inclusion in one genetic species, for which the name Xanthomonas campestris is proposed. In addition, 60 to 80% of the Xanthomonas DNA hybridized with DNA from several Pseudomonas strains. These results are evidence of the close genetic relationship between the two genera.
德莱,J.(比利时根特大学)和S.弗里德曼。黄单胞菌属和假单胞菌属脱氧核糖核酸的相似性。《细菌学杂志》89:1306 - 1309。1965年。——用DNA - 琼脂法检测到,在黄单胞菌属内以及黄单胞菌属和假单胞菌属之间存在明显的脱氧核糖核酸(DNA)杂交现象。来自天竺葵黄单胞菌的经剪切、变性的C(14) - DNA与来自八个不同黄单胞菌命名种的高分子量、变性的琼脂结合DNA杂交率达80%至100%。这九个菌株中DNA的近乎一致性支持将它们归入一个遗传种,为此提议命名为野油菜黄单胞菌。此外,60%至80%的黄单胞菌DNA与几种假单胞菌菌株的DNA杂交。这些结果证明了这两个属之间存在密切的遗传关系。