不同条件对大肠杆菌中土霉素积累的影响
EFFECT OF VARIOUS CONDITIONS ON ACCUMULATION OF OXYTETRACYCLINE IN ESCHERICHIA COLI.
作者信息
IZAKI K, ARIMA K
出版信息
J Bacteriol. 1965 May;89(5):1335-9. doi: 10.1128/jb.89.5.1335-1339.1965.
Izaki, Kazuo (University of Tokyo, Tokyo, Japan), and Kei Arima. Effect of various conditions on accumulation of oxytetracycline in Escherichia coli. J. Bacteriol. 89:1335-1339. 1965.-Accumulation of large amounts of oxytetracycline occurred in Escherichia coli when the cells were incubated with high concentrations of oxytetracycline (100 to 400 mug/ml) in nutrient broth or in a medium containing glucose, K(2)HPO(4), and MgSO(4). In the absence of glucose or MgSO(4), the accumulation was very small. The optimal pH for accumulation was about 6.5. Manganous ion could replace Mg(++) in promoting the accumulation, though with decreased effectiveness. Malate and succinate were effective promoters of accumulation as well as glucose. Accumulation was inhibited at low temperatures or in the presence of metabolic inhibitors such as 2,4-dinitrophenol or sodium azide.
伊扎基，和夫（日本东京大学，东京），以及有马圭。不同条件对大肠杆菌中土霉素积累的影响。《细菌学杂志》89:1335 - 1339。1965年。——当细胞在营养肉汤中或在含有葡萄糖、磷酸氢二钾和硫酸镁的培养基中与高浓度土霉素（100至400微克/毫升）一起培养时，大肠杆菌中会积累大量土霉素。在没有葡萄糖或硫酸镁的情况下，积累量非常小。积累的最适pH约为6.5。锰离子在促进积累方面可以替代镁离子，尽管效果有所降低。苹果酸和琥珀酸以及葡萄糖都是积累的有效促进剂。在低温或存在代谢抑制剂如2,4 -二硝基苯酚或叠氮化钠的情况下，积累会受到抑制。
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