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核心技术专利：CN118964589B侵权必究

核心技术专利：CN118964589B侵权必究

Biochem J. 1971 Jun;123(2):267-73. doi: 10.1042/bj1230267.

A membrane fraction from Escherichia coli has been prepared essentially free from ribosomes by treatment of the membranes with Triton X-100 at 0 degrees C followed by differential centrifugation. 2. The ribosome-free membrane vesicles absorbed tetracycline by a reversible temperature-dependent process with an apparent K(m) of 0.029mm at pH7.5 and 37 degrees C. 3. The absorption process was negligible below 25 degrees C and had an optimum at 40 degrees C; a pH optimum at 7.5 was observed. 4. The absorption of tetracycline was strongly inhibited by EDTA and ATP; ADP inhibited less strongly and AMP had no effect. 5. There was no significant difference in the rates or extent of uptake of tetracycline by membranes prepared from tetracycline-sensitive and tetracycline-resistant, R-factor-bearing E. coli.

通过在0℃用Triton X-100处理膜，随后进行差速离心，制备了基本上不含核糖体的大肠杆菌膜部分。2. 无核糖体的膜囊泡通过可逆的温度依赖性过程吸收四环素，在pH7.5和37℃时表观K(m)为0.029mm。3. 在25℃以下吸收过程可忽略不计，在40℃时有最佳值；观察到pH最佳值为7.5。4. 四环素的吸收受到EDTA和ATP的强烈抑制；ADP抑制作用较弱，AMP无作用。5. 由四环素敏感和携带R因子的四环素抗性大肠杆菌制备的膜，在四环素摄取速率或程度上没有显著差异。

Biochem J. 1971 Jun;123(2):267-73. doi: 10.1042/bj1230267.

A membrane fraction from Escherichia coli has been prepared essentially free from ribosomes by treatment of the membranes with Triton X-100 at 0 degrees C followed by differential centrifugation. 2. The ribosome-free membrane vesicles absorbed tetracycline by a reversible temperature-dependent process with an apparent K(m) of 0.029mm at pH7.5 and 37 degrees C. 3. The absorption process was negligible below 25 degrees C and had an optimum at 40 degrees C; a pH optimum at 7.5 was observed. 4. The absorption of tetracycline was strongly inhibited by EDTA and ATP; ADP inhibited less strongly and AMP had no effect. 5. There was no significant difference in the rates or extent of uptake of tetracycline by membranes prepared from tetracycline-sensitive and tetracycline-resistant, R-factor-bearing E. coli.

通过在0℃用Triton X-100处理膜，随后进行差速离心，制备了基本上不含核糖体的大肠杆菌膜部分。2. 无核糖体的膜囊泡通过可逆的温度依赖性过程吸收四环素，在pH7.5和37℃时表观K(m)为0.029mm。3. 在25℃以下吸收过程可忽略不计，在40℃时有最佳值；观察到pH最佳值为7.5。4. 四环素的吸收受到EDTA和ATP的强烈抑制；ADP抑制作用较弱，AMP无作用。5. 由四环素敏感和携带R因子的四环素抗性大肠杆菌制备的膜，在四环素摄取速率或程度上没有显著差异。