嗜硒陶厄氏菌中用于亚硒酸盐和硝酸盐呼吸的末端还原酶是两种不同的酶。
The terminal reductases for selenate and nitrate respiration in Thauera selenatis are two distinct enzymes.
作者信息
Rech S A, Macy J M
机构信息
Department of Animal Science, University of California, Davis 95616.
出版信息
J Bacteriol. 1992 Nov;174(22):7316-20. doi: 10.1128/jb.174.22.7316-7320.1992.
Abstract
A number of approaches have been used to show that a recently isolated selenate-respiring bacterium, Thauera selenatis, is able to synthesize both a selenate reductase (SR) and a nitrate reductase (NR). (i) The pH optimum of the SR was found to be 6.0; that of the NR was 7.0. (ii) The presence of nitrate did not inhibit selenate reduction in selenate-grown cells. (iii) In cell extracts, the highest SR or NR activity was observed in cells grown with the respective electron acceptor. (iv) Mutants that were unable to grow with nitrate as the terminal electron acceptor and lacked NR activity were isolated; these mutants grew normally with selenate and synthesized SR. (v) The SR was found in the periplasmic space of the cell, whereas the NR was present in the cytoplasmic membrane. A hypothetical electron transport system involving the SR is described.
摘要
已采用多种方法来证明，最近分离出的一种能进行硒酸盐呼吸的细菌——硒酸盐陶厄氏菌（Thauera selenatis），能够合成硒酸盐还原酶（SR）和硝酸盐还原酶（NR）。（i）发现SR的最适pH值为6.0；NR的最适pH值为7.0。（ii）在以硒酸盐生长的细胞中，硝酸盐的存在并不抑制硒酸盐的还原。（iii）在细胞提取物中，在用相应电子受体生长的细胞中观察到最高的SR或NR活性。（iv）分离出了不能以硝酸盐作为末端电子受体生长且缺乏NR活性的突变体；这些突变体在硒酸盐存在下能正常生长并合成SR。（v）发现SR存在于细胞的周质空间中，而NR存在于细胞质膜中。描述了一个涉及SR的假想电子传递系统。
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