van 't Klooster G A, Kolker H J, Woutersen-van Nijnanten F M, Noordhoek J, van Miert A S
Department of Veterinary Basic Sciences, University of Utrecht, Netherlands.
J Chromatogr. 1992 Sep 2;579(2):354-60.
A high-performance liquid chromatographic method is presented for the determination of trimethoprim (TMP), 3'-hydroxy-TMP, 4'-hydroxy-TMP, alpha-hydroxy-TMP and two TMP N-oxides. The last two metabolites appear to decompose on liquid extraction. TMP and its oxidative metabolites are separated using a C18 radial-compression column and quantified by UV detection at 230 nm. Calibration curves are linear from 0.5 to at least 50 microM. The limit of detection is 0.05-0.15 micrograms/ml. In in vitro rat liver metabolism studies. 3'- and 4'-hydroxylation of TMP appear to be important metabolic pathways whereas TMP N-oxides are minor metabolites.
本文介绍了一种高效液相色谱法，用于测定甲氧苄啶（TMP）、3'-羟基-TMP、4'-羟基-TMP、α-羟基-TMP和两种TMP N-氧化物。后两种代谢物在液液萃取时似乎会分解。使用C18径向压缩柱分离TMP及其氧化代谢物，并通过230nm处的紫外检测进行定量。校准曲线在0.5至至少50μM范围内呈线性。检测限为0.05 - 0.15μg/ml。在体外大鼠肝脏代谢研究中，TMP的3'-和4'-羟基化似乎是重要的代谢途径，而TMP N-氧化物是次要代谢物。
