高效液相色谱法测定细胞培养基和微粒体孵育混合物中的甲氧苄啶及其氧化代谢产物
Determination of trimethoprim and its oxidative metabolites in cell culture media and microsomal incubation mixtures by high-performance liquid chromatography.
作者信息
van 't Klooster G A, Kolker H J, Woutersen-van Nijnanten F M, Noordhoek J, van Miert A S
机构信息
Department of Veterinary Basic Sciences, University of Utrecht, Netherlands.
出版信息
J Chromatogr. 1992 Sep 2;579(2):354-60.
A high-performance liquid chromatographic method is presented for the determination of trimethoprim (TMP), 3'-hydroxy-TMP, 4'-hydroxy-TMP, alpha-hydroxy-TMP and two TMP N-oxides. The last two metabolites appear to decompose on liquid extraction. TMP and its oxidative metabolites are separated using a C18 radial-compression column and quantified by UV detection at 230 nm. Calibration curves are linear from 0.5 to at least 50 microM. The limit of detection is 0.05-0.15 micrograms/ml. In in vitro rat liver metabolism studies. 3'- and 4'-hydroxylation of TMP appear to be important metabolic pathways whereas TMP N-oxides are minor metabolites.
本文介绍了一种高效液相色谱法，用于测定甲氧苄啶（TMP）、3'-羟基-TMP、4'-羟基-TMP、α-羟基-TMP和两种TMP N-氧化物。后两种代谢物在液液萃取时似乎会分解。使用C18径向压缩柱分离TMP及其氧化代谢物，并通过230nm处的紫外检测进行定量。校准曲线在0.5至至少50μM范围内呈线性。检测限为0.05 - 0.15μg/ml。在体外大鼠肝脏代谢研究中，TMP的3'-和4'-羟基化似乎是重要的代谢途径，而TMP N-氧化物是次要代谢物。
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