Gonzalez M J, Gray J I, Schemmel R A, Dugan L, Welsch C W
Department of Food Science and Human Nutrition, Michigan State University, East Lansing 48824.
J Nutr. 1992 Nov;122(11):2190-5. doi: 10.1093/jn/122.11.2190.
Purified corn and fish oil diets with different types and concentrations of antioxidants were evaluated for oxidation products. In addition, a determination of different organ and carcass oxidation product levels was performed. Peroxide value and thiobarbituric acid assays were performed on the diets immediately after mixing (0 h) and 24, 48 and 72 h after being fed to mice. The AIN-recommended level of antioxidant addition (butylated hydroxytoluene, 0.02 g/100 g oil) and even the addition of 100 times this level (2 g/100 g oil), although decreasing the level of oxidation products, failed to totally prevent oxidative deterioration in diets high in fish oil. Furthermore, other antioxidants added in excess to the fish oil diets also failed to completely suppress oxidative deterioration of the diets and, in addition, when fed daily to mice for a period of 4 wk, caused an accumulation of lipid peroxidation products in certain organs (e.g., heart, skeletal muscle, mammary glands) and in the carcass. These results provide evidence that in the preparation of fish oil diets, the addition of antioxidants at the AIN-recommended level, or even levels substantially higher, does not completely suppress oxidative deterioration of experimental diets.
对含有不同类型和浓度抗氧化剂的纯化玉米和鱼油日粮的氧化产物进行了评估。此外，还测定了不同器官和胴体的氧化产物水平。在日粮混合后立即（0小时）以及喂给小鼠24、48和72小时后，对日粮进行了过氧化值和硫代巴比妥酸测定。美国营养学会推荐的抗氧化剂添加水平（丁基羟基甲苯，0.02克/100克油），甚至是该水平的100倍（2克/100克油），尽管能降低氧化产物水平，但仍无法完全防止富含鱼油的日粮发生氧化变质。此外，在鱼油日粮中过量添加的其他抗氧化剂也未能完全抑制日粮的氧化变质，而且，当每天喂给小鼠4周时，会导致某些器官（如心脏、骨骼肌、乳腺）和胴体中脂质过氧化产物的积累。这些结果表明，在制备鱼油日粮时，按照美国营养学会推荐的水平添加抗氧化剂，甚至是大大高于该水平的添加量，都不能完全抑制实验日粮的氧化变质。
