HEED W B, KIRCHER H W
Science. 1965 Aug 13;149(3685):758-61. doi: 10.1126/science.149.3685.758.
Drosophila pachea, which breeds only in the stems of senita cactus (Lophocereus schottii) throughout the Sonoran Desert, requires the cactus as a dietary supplement when reared on laboratory media. Delta(7)-Stigmasten-3beta-ol, isolated from the cactus or synthesized, can replace the cactus in the diet of flies reared nonaseptically or axenically. Delta(7)-Cholesten-3beta-ol and Delta(5, 7)-cholestadien-3beta-ol could be substituted for the cactus sterol; Delta(5, 7)-stigmastadien-3beta-ol produced infertile females. Cholesterol, 4alpha-methyl-Delta(7)-cholesten-3beta3-ol, beta-sitosterol, stigmasterol, ergosterol, and Delta(7)-ergosten- 3beta-ol did not support larval growth.
帕切果蝇仅在索诺兰沙漠的senita仙人掌(Lophocereus schottii)茎中繁殖,在实验室培养基上饲养时,需要仙人掌作为饮食补充剂。从仙人掌中分离或合成的Delta(7)-豆甾-3β-醇可以替代仙人掌,用于非无菌或无菌饲养的果蝇饮食中。Delta(7)-胆甾-3β-醇和Delta(5, 7)-胆甾二烯-3β-醇可以替代仙人掌甾醇;Delta(5, 7)-豆甾二烯-3β-醇会产生不育雌性。胆固醇、4α-甲基-Delta(7)-胆甾-3β-醇、β-谷甾醇、豆甾醇、麦角甾醇和Delta(7)-麦角甾-3β-醇不支持幼虫生长。