WASSERMAN A E
Appl Microbiol. 1965 Mar;13(2):175-80. doi: 10.1128/am.13.2.175-180.1965.
Pigments produced by four species of Pseudomonas during growth in three media were examined for visible and ultraviolet absorption. Ultraviolet fluorescence excitation and emission spectra were obtained. In all pigments, an absorption maximum occurs at 405 mmu. Ultraviolet excitation of fluorescence occurs primarily at 400 to 410 mmu, with smaller maxima at 460 mmu, or 525 mmu, depending on pH, bacterial species, or medium. Emission maxima, after excitation at 410 mmu, occur at 390 mmu, and 455 to 475 mmu. The differences in fluorescence spectra may be used for taxonomic classification of the Pseudomonas.
对四种假单胞菌在三种培养基中生长期间产生的色素进行了可见吸收和紫外吸收检测。获得了紫外荧光激发光谱和发射光谱。在所有色素中,最大吸收峰出现在405毫微米处。荧光的紫外激发主要发生在400至410毫微米处,根据pH值、细菌种类或培养基的不同,在460毫微米或525毫微米处有较小的最大值。在410毫微米激发后,发射最大值出现在390毫微米以及455至475毫微米处。荧光光谱的差异可用于假单胞菌的分类。