MacDonald J C, Bishop G G
Biochim Biophys Acta. 1984 Jul 16;800(1):11-20. doi: 10.1016/0304-4165(84)90088-6.
The major chromophore of a mixture of fluorescent pigments produced by Pseudomonas aeruginosa ATCC 9027 had pH-dependent absorption, excitation, and emission spectra, such that two ionic forms existed in the ground state and three in the excited states. The pigments could complex with several metal ions to change fluorescence and absorption spectra. Although the pigments were separable into several components, spectra indicated that the same fluorescent chromophore was present in each component. Hydrolysis of the mixture of pigments gave amino acids which did not include alanine or lysine. These pigments must therefore differ from those described by other workers, even though similarities of the chromophores were evident from comparisons with data in the literature, and from comparisons of a hydrolytic product of the mixture of pigments, termed compound F, with the chromophore of the fluorescent pigment of Azotobacter vinelandii. Drastic hydrolysis of the latter chromophore also yielded compound F.
铜绿假单胞菌ATCC 9027产生的荧光色素混合物的主要发色团具有pH依赖性的吸收、激发和发射光谱，使得在基态存在两种离子形式，在激发态存在三种离子形式。这些色素可与几种金属离子络合以改变荧光和吸收光谱。尽管这些色素可分离成几个组分，但光谱表明每个组分中都存在相同的荧光发色团。色素混合物的水解产生了不包括丙氨酸或赖氨酸的氨基酸。因此，这些色素必定与其他研究者描述的色素不同，尽管从与文献数据的比较以及将色素混合物的水解产物（称为化合物F）与棕色固氮菌荧光色素的发色团进行比较中可以明显看出发色团的相似性。后一种发色团的剧烈水解也产生了化合物F。
