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Subunit requirements for Torpedo AChR channel expression: a specific role for the delta-subunit in voltage-dependent gating.

作者信息

Golino M D, Hamill O P

机构信息

Section of Neurobiology and Behavior, Cornell University, Ithaca, New York 14853.

出版信息

J Membr Biol. 1992 Sep;129(3):297-309. doi: 10.1007/BF00232911.

DOI:10.1007/BF00232911
PMID:1433281
Abstract

This study examines the subunit requirement for Torpedo acetylcholine receptor (AChR) channel expression and the influence of non-alpha-subunit deletions on single AChR-channel currents. Xenopus oocytes injected with subunit combinations deficient in single non-alpha-subunit mRNA transcripts display the following order of ACh sensitivity: beta-less > gamma-less > delta-less. Oocytes injected with only the alpha-subunit and one non-alpha-subunit display the order: alpha delta > alpha gamma > alpha beta. These sequences indicate the effectiveness of non-alpha-subunit substitution is delta > gamma > beta. Single AChR-channel currents measured in oocytes deficient in either beta or gamma display conductance and voltage-sensitive burst kinetics similar to the wild-type channel. In contrast, the delta-less combination express channels with burst kinetics that are relatively faster and voltage insensitive. These results indicate that either a specific structural domain in the delta-subunit or its specific interactions with the alpha-subunit contribute to the voltage-dependent gating of the Torpedo AChR channel.

摘要

相似文献

1
Subunit requirements for Torpedo AChR channel expression: a specific role for the delta-subunit in voltage-dependent gating.
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2
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本文引用的文献

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Acetylcholine receptor: complex of homologous subunits.乙酰胆碱受体：同源亚基复合体。
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Equilibrium properties of mouse-Torpedo acetylcholine receptor hybrids expressed in Xenopus oocytes.在非洲爪蟾卵母细胞中表达的小鼠-电鳐乙酰胆碱受体杂合体的平衡特性。
J Gen Physiol. 1987 Oct;90(4):553-73. doi: 10.1085/jgp.90.4.553.
9
Arrangement of the acetylcholine receptor subunits in the resting and desensitized states, determined by cryoelectron microscopy of crystallized Torpedo postsynaptic membranes.通过对电鳐突触后膜晶体进行冷冻电子显微镜观察确定乙酰胆碱受体亚基在静息和脱敏状态下的排列方式。
J Cell Biol. 1988 Sep;107(3):1123-38. doi: 10.1083/jcb.107.3.1123.
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Control of Torpedo acetylcholine receptor biosynthesis in Xenopus oocytes.非洲爪蟾卵母细胞中鱼雷乙酰胆碱受体生物合成的调控
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