用非酶法制备5'-三磷酸腺苷
THE PREPARATION OF ADENOSINE 5'-PYROPHOSPHATE BY A NON-ENZYMIC METHOD.
作者信息
DAWSON R M, FORD M, EICHBERG J
出版信息
Biochem J. 1965 Apr;95(1):104-6. doi: 10.1042/bj0950104.
Abstract
- A non-enzymic method for the preparation of adenosine 5'-diphosphate is described, in which the terminal phosphate of adenosine 5'-triphosphate is transferred to methanol in the presence of hydrochloric acid. The final purified product can be obtained in 60% yield. 2. Experiments with [(14)C]methanol showed that no methylation of the adenosine diphosphate occurs during the reaction. 3. Confirmation that the pyrophosphate moiety of the adenosine diphosphate produced was in the 5'-position was obtained by: (a) periodate oxidation; (b) treatment with apyrase and examination of the resulting adenylic acid isomer by paper chromatography. 4. The method appears to be generally applicable to the preparation of nucleoside 5'-diphosphates from the corresponding nucleoside 5'-triphosphates.
摘要
- 描述了一种制备5'-二磷酸腺苷的非酶法，其中在盐酸存在下，5'-三磷酸腺苷的末端磷酸基团转移至甲醇。最终纯化产物的产率可达60%。2. 用[(14)C]甲醇进行的实验表明，反应过程中未发生二磷酸腺苷的甲基化。3. 通过以下方法证实了所生成的二磷酸腺苷的焦磷酸部分位于5'-位：(a) 高碘酸盐氧化；(b) 用腺苷三磷酸双磷酸酶处理，并通过纸色谱法检查所得的腺苷酸异构体。4. 该方法似乎普遍适用于从相应的5'-三磷酸核苷制备5'-二磷酸核苷。
相似文献
1
THE PREPARATION OF ADENOSINE 5'-PYROPHOSPHATE BY A NON-ENZYMIC METHOD.用非酶法制备5'-三磷酸腺苷
Biochem J. 1965 Apr;95(1):104-6. doi: 10.1042/bj0950104.
2
Periodate oxidations of enamines. I. Oxidation of adenosine 5'-monophosphate in the presence of methylamine.烯胺的高碘酸盐氧化反应。I. 在甲胺存在下5'-单磷酸腺苷的氧化反应
Biochemistry. 1971 Dec 7;10(25):4699-705. doi: 10.1021/bi00801a016.
3
POTENTIOMETRIC STUDIES OF THE SECONDARY PHOSPHATE IONIZATIONS OF AMP, ADP, AND ATP, AND CALCULATIONS OF THERMODYNAMIC DATA FOR THE HYDROLYSIS REACTIONS.AMP、ADP和ATP二级磷酸电离的电位滴定研究以及水解反应热力学数据的计算
Biochemistry. 1963 May-Jun;2:501-8. doi: 10.1021/bi00903a019.
4
The reaction of methylamine with periodate-oxidized adenosine 5'-phosphate.甲胺与高碘酸盐氧化的腺苷5'-磷酸的反应。
Biochemistry. 1963 Mar-Apr;2:344-50. doi: 10.1021/bi00902a029.
5
PURIFICATION AND SOME PROPERTIES OF ERYTHROCYTE ACID PHOSPHO-MONOESTERASE.红细胞酸性磷酸单酯酶的纯化及某些性质
J Biochem. 1964 Mar;55:321-6. doi: 10.1093/oxfordjournals.jbchem.a127886.
6
[Structure of electrochemically reduced adenine nucleotides. Their interaction with inorganic phosphate].[电化学还原腺嘌呤核苷酸的结构。它们与无机磷酸盐的相互作用]
Biokhimiia. 1976 Sep;41(9):1561-6.
7
Formation of adenosine triphosphate in the oxidation of a model for the reduced pyridine nucleotides.还原型吡啶核苷酸模型氧化过程中三磷酸腺苷的形成。
Biochemistry. 1972 Jul 4;11(14):2606-10. doi: 10.1021/bi00764a009.
8
[THE PROPERTIES OF P-1-(ADENOSINE-5')-DIPHOSPHO-(P2-- N)-PHENYLALANINE METHYL ESTER].[P-1-(腺苷-5')-二磷酸-(P2--N)-苯丙氨酸甲酯的性质]
Biokhimiia. 1964 Jul-Aug;29:690-6.
9
Electrochromatographic separation of inorganic phosphate, adenosine monophosphate, adenosine diphosphate, and adenosine triphosphate.无机磷酸盐、一磷酸腺苷、二磷酸腺苷和三磷酸腺苷的电色谱分离。
Anal Biochem. 1963 Jun;5:542-7. doi: 10.1016/0003-2697(63)90074-5.
10
Orthophosphate analysis by the Fiske-SubbaRow method and interference by adenosine phosphates and pyrophosphate at variable acid pH.采用Fiske-SubbaRow法进行正磷酸盐分析以及在可变酸性pH条件下腺苷磷酸和焦磷酸的干扰
Anal Biochem. 1973 Dec;56(2):566-70. doi: 10.1016/0003-2697(73)90221-2.
本文引用的文献
1
Myosin and adenosinetriphosphatase.肌球蛋白与三磷酸腺苷酶
Biochem J. 1942 Feb;36(1-2):121-39. doi: 10.1042/bj0360121.
2
Chromatographic studies of nucleic acids; a technique for the identification and estimation of purine and pyrimidine bases, nucleosides and related substances.核酸的色谱研究；一种用于嘌呤和嘧啶碱基、核苷及相关物质的鉴定和定量的技术。
Biochem J. 1949;45(3):294-8. doi: 10.1042/bj0450294.
3
The contribution of Alban G. Smith, of Kentucky, to the early history of surgery.肯塔基州的阿尔班·G·史密斯对外科早期历史的贡献。
J Ky State Med Assoc. 1951 Sep;49(9):398-401.
4
The preparation of adenosinetriphosphate from acetone-dried muscle and a chemical method for the preparation of adenosinediphosphate.从丙酮干燥肌肉中制备三磷酸腺苷以及制备二磷酸腺苷的化学方法。
Biochem J. 1950 Jun-Jul;47(1):105-9. doi: 10.1042/bj0470105.
5
THE NON-ENZYMATIC TRANSPHOSPHORYLATION OF LOWER ALIPHATIC ALCOHOLS BY ADENOSINE TRIPHOSPHATE.三磷酸腺苷对低级脂肪醇的非酶转磷酸作用
Biochim Biophys Acta. 1964 Nov 8;93:425-6. doi: 10.1016/0304-4165(64)90397-6.
6
The effect of bivalent metal ions on the hydrolysis of adenosine di- and triphosphate.二价金属离子对二磷酸腺苷和三磷酸腺苷水解的影响。
Biochemistry. 1963 Mar-Apr;2:350-7. doi: 10.1021/bi00902a030.
7
The isolation of a new lipid, triphosphoinositide, and monophosphoinositide from ox brain.从牛脑中分离出一种新的脂质——三磷酸肌醇和单磷酸肌醇。
Biochem J. 1961 Dec;81(3):535-40. doi: 10.1042/bj0810535.
8
Improvements in the method of determining individual phospholipids in a complex mixture by successive chemical hydrolyses.通过连续化学水解测定复杂混合物中单个磷脂方法的改进。
Biochem J. 1962 Sep;84(3):497-501. doi: 10.1042/bj0840497.
9
Techniques in tissue metabolism. 4. Apparatus for maintaining and rapidly transferring tissue sections.组织代谢技术。4. 用于保存和快速转移组织切片的仪器。
Biochem J. 1956 Jun;63(2):231-5. doi: 10.1042/bj0630231.
Zotero 插件