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Biochem J. 1965 Apr;95(1):104-6. doi: 10.1042/bj0950104.
2
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本文引用的文献

1
Myosin and adenosinetriphosphatase.肌球蛋白与三磷酸腺苷酶
Biochem J. 1942 Feb;36(1-2):121-39. doi: 10.1042/bj0360121.
2
Chromatographic studies of nucleic acids; a technique for the identification and estimation of purine and pyrimidine bases, nucleosides and related substances.核酸的色谱研究；一种用于嘌呤和嘧啶碱基、核苷及相关物质的鉴定和定量的技术。
Biochem J. 1949;45(3):294-8. doi: 10.1042/bj0450294.
3
The contribution of Alban G. Smith, of Kentucky, to the early history of surgery.肯塔基州的阿尔班·G·史密斯对外科早期历史的贡献。
J Ky State Med Assoc. 1951 Sep;49(9):398-401.
4
The preparation of adenosinetriphosphate from acetone-dried muscle and a chemical method for the preparation of adenosinediphosphate.从丙酮干燥肌肉中制备三磷酸腺苷以及制备二磷酸腺苷的化学方法。
Biochem J. 1950 Jun-Jul;47(1):105-9. doi: 10.1042/bj0470105.
5
THE NON-ENZYMATIC TRANSPHOSPHORYLATION OF LOWER ALIPHATIC ALCOHOLS BY ADENOSINE TRIPHOSPHATE.三磷酸腺苷对低级脂肪醇的非酶转磷酸作用
Biochim Biophys Acta. 1964 Nov 8;93:425-6. doi: 10.1016/0304-4165(64)90397-6.
6
The effect of bivalent metal ions on the hydrolysis of adenosine di- and triphosphate.二价金属离子对二磷酸腺苷和三磷酸腺苷水解的影响。
Biochemistry. 1963 Mar-Apr;2:350-7. doi: 10.1021/bi00902a030.
7
The isolation of a new lipid, triphosphoinositide, and monophosphoinositide from ox brain.从牛脑中分离出一种新的脂质——三磷酸肌醇和单磷酸肌醇。
Biochem J. 1961 Dec;81(3):535-40. doi: 10.1042/bj0810535.
8
Improvements in the method of determining individual phospholipids in a complex mixture by successive chemical hydrolyses.通过连续化学水解测定复杂混合物中单个磷脂方法的改进。
Biochem J. 1962 Sep;84(3):497-501. doi: 10.1042/bj0840497.
9
Techniques in tissue metabolism. 4. Apparatus for maintaining and rapidly transferring tissue sections.组织代谢技术。4. 用于保存和快速转移组织切片的仪器。
Biochem J. 1956 Jun;63(2):231-5. doi: 10.1042/bj0630231.

用非酶法制备5'-三磷酸腺苷

THE PREPARATION OF ADENOSINE 5'-PYROPHOSPHATE BY A NON-ENZYMIC METHOD.

作者信息

DAWSON R M, FORD M, EICHBERG J

出版信息

Biochem J. 1965 Apr;95(1):104-6. doi: 10.1042/bj0950104.

DOI:10.1042/bj0950104
PMID:14333545
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1215183/
Abstract
  1. A non-enzymic method for the preparation of adenosine 5'-diphosphate is described, in which the terminal phosphate of adenosine 5'-triphosphate is transferred to methanol in the presence of hydrochloric acid. The final purified product can be obtained in 60% yield. 2. Experiments with [(14)C]methanol showed that no methylation of the adenosine diphosphate occurs during the reaction. 3. Confirmation that the pyrophosphate moiety of the adenosine diphosphate produced was in the 5'-position was obtained by: (a) periodate oxidation; (b) treatment with apyrase and examination of the resulting adenylic acid isomer by paper chromatography. 4. The method appears to be generally applicable to the preparation of nucleoside 5'-diphosphates from the corresponding nucleoside 5'-triphosphates.
摘要
  1. 描述了一种制备5'-二磷酸腺苷的非酶法，其中在盐酸存在下，5'-三磷酸腺苷的末端磷酸基团转移至甲醇。最终纯化产物的产率可达60%。2. 用[(14)C]甲醇进行的实验表明，反应过程中未发生二磷酸腺苷的甲基化。3. 通过以下方法证实了所生成的二磷酸腺苷的焦磷酸部分位于5'-位：(a) 高碘酸盐氧化；(b) 用腺苷三磷酸双磷酸酶处理，并通过纸色谱法检查所得的腺苷酸异构体。4. 该方法似乎普遍适用于从相应的5'-三磷酸核苷制备5'-二磷酸核苷。