镉对肾皮质和肝脏微粒体混合功能氧化酶活性影响的比较研究。

A comparative study of the effects of cadmium on microsomal mixed-function oxidase activities between kidney cortex and liver.

作者信息

Ding H P, Liu Y G

机构信息

Department of Environmental Toxicology, Tongji Medical University, Wuhan.

出版信息

J Tongji Med Univ. 1992;12(2):85-9. doi: 10.1007/BF02887786.

DOI:10.1007/BF02887786

PMID:1433423

Abstract

The effects of cadmium and phenobarbital (PB) administration on the activities of microsomal mixed-function oxidase (MFO) in rat kidney cortex and liver were studied. Five indices, including cytochrome P-450 contents, aniline hydroxylase, NADPH-cytochrome c reductase, aminopyrine N-demethylase and 7-ethoxycoumarin O-deethylase activities, were assayed. By comparison, it was found that PB could induce MFO activities in kidney cortex microsomes and liver microsomes, both hepatic and renal microsomal MFO were decreased by the ip injection of CdCl2, and Cd2+ could markedly decrease the phenobarbital induction to hepatic and renal microsomal MFO activities with the exception of NADPH-cytochrome c reductase in kidney cortex microsomes.

摘要

研究了给予镉和苯巴比妥（PB）对大鼠肾皮质和肝脏微粒体混合功能氧化酶（MFO）活性的影响。测定了五个指标，包括细胞色素P-450含量、苯胺羟化酶、NADPH-细胞色素c还原酶、氨基比林N-脱甲基酶和7-乙氧基香豆素O-脱乙基酶活性。通过比较发现，PB可诱导肾皮质微粒体和肝脏微粒体中的MFO活性，腹腔注射CdCl2可使肝脏和肾脏微粒体中的MFO均降低，并且除肾皮质微粒体中的NADPH-细胞色素c还原酶外，Cd2+可显著降低苯巴比妥对肝脏和肾脏微粒体MFO活性的诱导作用。