Muto A, Yokota T
Department of Molecular and Developmental Biology, University of Tokyo.
Nihon Rinsho. 1992 Aug;50(8):1781-6.
Interleukin 3 (IL-3) plays a critical role in growth and differentiation of myeloid cells. The human IL-3 gene, located on chromosome 5 contains several cis-acting DNA sequences, i.e. CLE (conserved lymphokine element) and a GC rich region, similar to the GM-CSF gene. The expression of the GM-CSF and IL-3 genes in activated T cells may be regulated coordinately through these cis-regulatory elements. Recently, reconstitution experiments using isolated cDNA clones for receptor subunits have revealed that two distinct proteins are required for the high affinity receptors for GM-CSF, IL-3 and IL-5; the alpha subunits bind with the corresponding ligands with low affinity, and the beta subunit is shared in common. Tyrosine kinase activity appears to be involved in the signal transduction of the IL-3 and GM-CSF receptors.
白细胞介素3（IL-3）在髓系细胞的生长和分化中起关键作用。位于5号染色体上的人类IL-3基因包含几个顺式作用DNA序列，即保守淋巴因子元件（CLE）和一个富含GC的区域，类似于GM-CSF基因。GM-CSF和IL-3基因在活化T细胞中的表达可能通过这些顺式调节元件进行协调调控。最近，使用分离的受体亚基cDNA克隆进行的重组实验表明，GM-CSF、IL-3和IL-5的高亲和力受体需要两种不同的蛋白质；α亚基与相应配体以低亲和力结合，β亚基是共用的。酪氨酸激酶活性似乎参与了IL-3和GM-CSF受体的信号转导。
