Hattori T, Furuta K, Nagao T, Nagamatsu T, Ito M, Suzuki Y
Department of Pharmacology, Faculty of Pharmacy, Meijo University, Nagoya, Japan.
Jpn J Pharmacol. 1992 Jun;59(2):159-69. doi: 10.1254/jjp.59.159.
The present study was conducted to investigate the antinephritic effects of berberine and coptisine, which are contained in Coptidis rhizoma, on original-type anti-GBM nephritis in rats. Berberine and coptisine at the doses of 0.5, 1.0 and 5.0 mg/kg/day, i.p. were effective in inhibiting urinary protein excretion, elevation of serum cholesterol and creatinine contents as well as glomerular histopathological changes. In addition, berberine at 20 mg/kg/day, p.o. also inhibited urinary protein excretion throughout the experimental periods. Berberine and coptisine inhibited platelet aggregation in both in vitro and in vivo assays, and berberine inhibited the decline of renal blood flow. Although berberine inhibited an increase in thromboxane B2 formation, it increased the formation of 6-keto-prostaglandin F1 alpha in platelets and isolated glomeruli. These results indicate that the antinephritic effects of berberine and coptisine may be partly due to antiplatelet action and improved renal hemodynamics via changing prostanoid synthesis.
本研究旨在探讨黄连根茎中所含的小檗碱和黄连碱对大鼠原型抗肾小球基底膜（GBM）肾炎的抗肾炎作用。腹腔注射剂量为0.5、1.0和5.0mg/kg/天的小檗碱和黄连碱可有效抑制尿蛋白排泄、血清胆固醇和肌酐含量升高以及肾小球组织病理学变化。此外，口服剂量为20mg/kg/天的小檗碱在整个实验期间也能抑制尿蛋白排泄。小檗碱和黄连碱在体外和体内试验中均能抑制血小板聚集，小檗碱还能抑制肾血流量下降。虽然小檗碱抑制血栓素B2生成增加，但它能增加血小板和分离肾小球中6-酮-前列腺素F1α的生成。这些结果表明，小檗碱和黄连碱的抗肾炎作用可能部分归因于抗血小板作用以及通过改变前列腺素合成改善肾血流动力学。
