表皮生长因子对培养的脑神经元的神经营养作用被蛋白激酶抑制剂阻断。

Neurotrophic effects of epidermal growth factor on cultured brain neurons are blocked by protein kinase inhibitors.

作者信息

Abe K, Takayanagi M, Saito H

机构信息

Department of Chemical Pharmacology, Faculty of Pharmaceutical Sciences, University of Tokyo, Japan.

出版信息

Jpn J Pharmacol. 1992 Jun;59(2):259-61. doi: 10.1254/jjp.59.259.

DOI:10.1254/jjp.59.259

PMID:1434124

Abstract

The influences of protein kinase inhibitors, K-252a and staurosporine, on the neurotrophic effects of epidermal growth factor (EGF) were investigated in dissociated cell cultures of the hippocampus and cerebellum of fetal rats. Addition of 1 ng/ml EGF enhanced the survival of cultured neurons of both brain regions. Both K-252a (10-200 nM) and staurosporine (1-100 nM) blocked the survival-promoting effects of EGF in a concentration-dependent manner. These results suggest that activation of protein kinase(s) is involved in the neurotrophic effects of EGF.

摘要

在胎鼠海马和小脑的解离细胞培养物中，研究了蛋白激酶抑制剂K-252a和星形孢菌素对表皮生长因子（EGF）神经营养作用的影响。添加1 ng/ml EGF可提高两个脑区培养神经元的存活率。K-252a（10 - 200 nM）和星形孢菌素（1 - 100 nM）均以浓度依赖性方式阻断EGF的促存活作用。这些结果表明蛋白激酶的激活参与了EGF的神经营养作用。