HIRAMOTO Y
J Cell Biol. 1965 Apr;25(1):SUPPL:161-7. doi: 10.1083/jcb.25.1.161.
A large quantity of paraffin oil, sucrose solution, or sea water was injected into the eggs of the heart urchin Clypeaster japonicus shortly before the onset of the first cleavage. The injected oil became spherical, pushing the mitotic apparatus aside. The sucrose solution mixed with the protoplasm and caused disintegration of the mitotic apparatus, and the sea water formed a vacuole at the center of the cell. In all these cases, cleavage may take place almost normally in spite of the absence of the mitotic apparatus or its displacement within the cell. In some eggs, furrowing may take place when more than fifty per cent of the endoplasm has been replaced with sea water before onset of cleavage.
在日本心形海胆(Clypeaster japonicus)首次卵裂开始前不久,向其卵内注入大量石蜡油、蔗糖溶液或海水。注入的油变成球形,将有丝分裂器推到一边。蔗糖溶液与原生质混合,导致有丝分裂器解体,海水在细胞中心形成一个液泡。在所有这些情况下,尽管细胞内没有有丝分裂器或其发生了移位,但卵裂仍可能几乎正常进行。在一些卵中,在卵裂开始前,当超过50%的内质被海水取代时,仍可能发生卵裂沟。