Sarikaputi M, Morimatsu M, Yamamoto S, Syuto B, Saito M, Naiki M
Department of Biochemistry, Faculty of Veterinary Medicine, Hokkaido University, Sapporo, Japan.
Jpn J Vet Res. 1992 May;40(1-2):1-12.
A semi-quantitative latex agglutination test for bovine serum CRP levels has been established by mixing diluted serum (or diluted standard serum) with a 1% latex suspension containing 0.489 micron latex particles coated with affinity-purified antibody at a ratio of 20 micrograms/mg latex. The agglutination was performed on a glass slide in a moist chamber at room temperature with 45 min. incubation. This test is reliable, reproducible and the results correlate with those of the single radial immunodiffusion (SRID) test. The effect of low temperature storage on CRP concentration revealed a 30% degradation of CRP during 2 years storage at 4 degrees C. The possible role of EDTA addition to prevent a decrease in serum CRP concentration by freezing and thawing is also discussed.
通过将稀释血清（或稀释标准血清）与含0.489微米乳胶颗粒的1%乳胶悬液以20微克/毫克乳胶的比例混合，已建立了一种用于检测牛血清CRP水平的半定量乳胶凝集试验。该乳胶悬液中的乳胶颗粒包被有亲和纯化抗体。凝集反应在室温下的湿盒中于载玻片上进行，孵育45分钟。该试验可靠、可重复，且结果与单向放射免疫扩散（SRID）试验的结果相关。低温储存对CRP浓度的影响显示，在4℃储存2年期间，CRP降解了30%。文中还讨论了添加EDTA以防止冻融导致血清CRP浓度降低的可能作用。
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