Tagata K, Yokoyama S, Ginbo T, Honda M, Okimura T, Odakura M, Nomura M, Yamamoto S
Tagata Animal Hospital, Shimane, Japan.
Vet Res Commun. 1996;20(1):21-30. doi: 10.1007/BF00346573.
A capillary reversed passive latex agglutination test (capillary RPLA) was developed which allows quantification of serum C-reactive protein (CRP) within approximately 15 min. The logarithmic regression line (calibration curve) obtained after measuring each CRP concentration three times in twofold dilutions of a standard canine serum containing 222 micrograms/ml of CRP was y = 6.394 + 0.030x (r = 0.995). Capillary RPLA permitted quantification of CRP in the range 6.9-222 micrograms/ml. The coefficients of variation ranged from 10.28% to 12.40%. The recovery rates (percentage recovery) of CRP by capillary RPLA were within the range 87% to 106%. On measuring the CRP concentrations in sera from 78 dogs by capillary RPLA, single radial immunodiffusion (SRID) and enzyme-linked immunosorbent assay (ELISA), close correlations were demonstrated between SRID and capillary RPLA (y = 7.250 + 1.109x, r = 0.978), between SRID and ELISA (y = 3.042 + 1.059x, r = 0.967), and between capillary RPLA and ELISA (y = 1.778 + 0.929x, r = 0.962). Capillary RPLA may be considered useful as a routine biochemical technique for measurement of serum CRP concentration in the dog.
开发了一种毛细管反向被动乳胶凝集试验（毛细管RPLA），可在约15分钟内对血清C反应蛋白（CRP）进行定量。在对含有222微克/毫升CRP的标准犬血清进行两倍稀释后，对每个CRP浓度进行三次测量，得到的对数回归线（校准曲线）为y = 6.394 + 0.030x（r = 0.995）。毛细管RPLA能够对6.9 - 222微克/毫升范围内的CRP进行定量。变异系数在10.28%至12.40%之间。通过毛细管RPLA测得的CRP回收率（回收百分比）在87%至106%范围内。通过毛细管RPLA、单向辐射免疫扩散法（SRID）和酶联免疫吸附测定法（ELISA）对78只犬的血清CRP浓度进行测量，结果表明SRID与毛细管RPLA之间（y = 7.250 + 1.109x，r = 0.978）、SRID与ELISA之间（y = 3.042 + 1.059x，r = 0.967）以及毛细管RPLA与ELISA之间（y = 1.778 + 0.929x，r = 0.962）均具有密切相关性。毛细管RPLA可被视为一种用于测量犬血清CRP浓度的常规生化技术。
