Stein G I, Kudryavtsev B N
Institute of Cytology, Russian Academy of Sciences, St-Petersburg.
J Theor Biol. 1992 Jun 7;156(3):349-63. doi: 10.1016/s0022-5193(05)80680-0.
A method for investigating weakly-proliferating cell populations of liver parenchyma on the basis of a quantitative analysis of hepatocyte polyploidization during postnatal development is described. The method uses a mathematical model which characterizes the hepatocyte polyploidization process, and incorporates data concerning the time course for relative frequencies of hepatocytes in different ploidy classes. As a result of these measurements and calculations for rat liver, transition rates of hepatocytes (the relative number of cells during a given time unit) from one ploidy class to another, and a coefficient for the reduction of hepatocyte mitotic activity with an increase in its ploidy class were obtained. Calculated curves show a good correspondence with the real process of hepatocyte frequency changes as they relate to changes in the age of the animals. To check this method, experiments investigating time changes of autoradiographic label content in the different ploidy classes of hepatocytes were carried out. By mathematically modeling the label diluting process resulting from cell proliferation and polyploidization, transition rates of hepatocytes were calculated, and they reflect values calculated from the model according to changes in occurrence frequencies.
描述了一种基于对出生后发育过程中肝细胞多倍体化的定量分析来研究肝实质弱增殖细胞群体的方法。该方法使用一个数学模型来表征肝细胞多倍体化过程，并纳入了不同倍性类别的肝细胞相对频率随时间变化的数据。通过对大鼠肝脏进行这些测量和计算，获得了肝细胞从一个倍性类别到另一个倍性类别的转变率（给定时间单位内细胞的相对数量），以及随着倍性类别增加肝细胞有丝分裂活性降低的系数。计算曲线与肝细胞频率随动物年龄变化的实际过程显示出良好的对应关系。为了检验该方法，进行了研究不同倍性类别的肝细胞放射自显影标记物含量随时间变化的实验。通过对细胞增殖和多倍体化导致的标记物稀释过程进行数学建模，计算出肝细胞的转变率，这些转变率反映了根据出现频率变化从模型计算出的值。
