Sawicka E
Histochemistry. 1977 Sep 22;53(4):327-39. doi: 10.1007/BF00509249.
The ultrahistochemical localization of the "reversed" ATPase activity was investigated. Red muscle fibres showed permanent sarcomere contraction, enzymatic activity in the inner membrane and matrix of mitochondria, and large, osmiophilic, probably calcium-containing structures within mitochondria and on their outside. White muscle fibre sarcomeres were relaxed, and activity within their sarcoplasmic reticulum was marked, but slight in the mitochondria. The relaxed state of the sarcomere in the white muscle fibres is supposed to be connected with inactivation of myofibrillar ATPase by acid preincubation, whereas red muscle contraction indicates that acid preincubation does not inactivate their myofibrillar ATPase. That the product of its activity failed to become visible in the sarcomeres is probably due to imperfection of the method. Two sub-types of red muscle fibres were distinguished: those showing only enzymatic activity in mitochondria, and those containing large intra- and extramitochondrial osmiophilic structures. The origin and composition of these structures is difficult to explain. A relation seems to exist between their presence within mitochondria and outside.
对“反向”ATP酶活性进行了超组织化学定位研究。红色肌纤维呈现出永久性肌节收缩、线粒体内膜和基质中的酶活性，以及线粒体内及其外部存在的大的、嗜锇的、可能含钙的结构。白色肌纤维的肌节松弛，其肌浆网内的活性明显，但线粒体中的活性较弱。白色肌纤维中肌节的松弛状态被认为与酸预孵育导致肌原纤维ATP酶失活有关，而红色肌纤维的收缩表明酸预孵育不会使其肌原纤维ATP酶失活。其活性产物在肌节中未能显现，可能是由于该方法存在缺陷。区分出了两种红色肌纤维亚型：一种仅在线粒体中显示酶活性，另一种含有大的线粒体内外嗜锇结构。这些结构的起源和组成难以解释。它们在线粒体内外的存在之间似乎存在某种关系。
