Malpeli J G, Weyand T G, LaClair R
Department of Psychology, University of Illinois, Champaign 61820.
J Neurosci Methods. 1992 Aug;44(1):19-26. doi: 10.1016/0165-0270(92)90109-q.
A new method of mounting a microdrive on the skull and adjusting the trajectories of microelectrodes is described. The key to this system is a swiveling guide tube held in a small, skull-mounted base by a low-melting-point metal alloy. The microelectrode is advanced via a modified, commercially available, miniature microdrive screwed onto the guide tube. To change the trajectory of the electrode, the alloy is melted in place, and the guide tube swiveled to a different angle. The microelectrode or the trajectory of the pass may be changed in a few minutes while easily maintaining aseptic conditions. The entire assembly is small enough so that several can be simultaneously implanted on the skull of a cat. A metal crown is used to fix the head during recording sessions or to hold a fiberglass cap that protects all skull implants between sessions. Since the microdrives need not be removed between recording sessions, an electrode pass may be continued from day to day. Although guide tubes are generally employed only for subcortical structures, this arrangement also works well when recording from cortex in deeper parts of gyri.
本文描述了一种将微驱动器安装在颅骨上并调整微电极轨迹的新方法。该系统的关键在于一个可旋转的导管，它通过低熔点金属合金固定在一个安装在颅骨上的小型基座中。微电极通过拧在导管上的经过改进的市售微型微驱动器推进。为了改变电极的轨迹，合金在原位熔化，导管旋转到不同的角度。在轻松保持无菌条件的同时，几分钟内即可改变微电极或穿刺轨迹。整个组件体积小，因此可以同时植入几只猫的颅骨上。在记录过程中，使用金属帽固定头部，或者在不同记录时段之间固定一个玻璃纤维帽，以保护所有颅骨植入物。由于在记录时段之间无需移除微驱动器，因此电极穿刺可以日复一日地持续进行。虽然导管通常仅用于皮质下结构，但当从脑回较深部位的皮质进行记录时，这种布置也能很好地发挥作用。
