Sakakura C, Takahashi T, Sawai K, Hagiwara A, Yamane T, Itoh M, Sasabe T, Lee M, Shobayashi S
First Department of Surgery, Kyoto Prefectural University of Medicine, Japan.
Lymphology. 1992 Sep;25(3):114-9.
The effect of local tissue injection of activated carbon particle adsorbing aclacinomycin A (ACR-CH) on the cytotoxicity in popliteal, inguinal, paraaortic, and axillary lymph nodes was investigated in mice. Aclacinomycin (0.2 mg/kg), a potent antineoplastic drug was injected subcutaneously into the footpad of mice in the form of ACR-CH or as an ACR solution. After a single injection of ACR-CH, the regional nodal cytotoxic response against mouse YAC-1 lymphoma cells was markedly increased and sustained for 7-10 days. The immune response was also increased after ACR solution but to a much lesser extent. These effects were found in popliteal, inguinal, and paraaortic lymph nodal effector cells but not in the more remote axillary nodes. Absorption of adherence cells largely abrogated the cytotoxic response. These results suggest that ACR-CH did not impair but rather stimulated nodal immunoregulatory cells. Potentially ACR-CH may enhance immune responsiveness of regional lymph nodes after subcutaneous administration while concomitantly curtailing neoplastic growth in these same lymph nodes.
在小鼠中研究了局部组织注射吸附阿克拉霉素A的活性炭颗粒（ACR-CH）对腘窝、腹股沟、主动脉旁和腋窝淋巴结细胞毒性的影响。将强效抗肿瘤药物阿克拉霉素（0.2mg/kg）以ACR-CH的形式或ACR溶液皮下注射到小鼠的足垫中。单次注射ACR-CH后，针对小鼠YAC-1淋巴瘤细胞的局部淋巴结细胞毒性反应显著增强，并持续7-10天。注射ACR溶液后免疫反应也增强，但程度要小得多。在腘窝、腹股沟和主动脉旁淋巴结效应细胞中发现了这些作用，但在较远的腋窝淋巴结中未发现。粘附细胞的吸收在很大程度上消除了细胞毒性反应。这些结果表明，ACR-CH并未损害而是刺激了淋巴结免疫调节细胞。ACR-CH可能在皮下给药后增强局部淋巴结的免疫反应性，同时抑制这些相同淋巴结中的肿瘤生长。
