通过密度梯度电泳法分离牛乳酪蛋白的主要成分
THE SEPARATION OF MAJOR COMPONENTS OF THE CASEIN OF BOVINE MILK BY ELECTROPHORESIS IN A DENSITY GRADIENT.
作者信息
MANSON W
出版信息
Biochem J. 1965 Feb;94(2):452-7. doi: 10.1042/bj0940452.
Abstract
- Zonal electrophoresis in a column stabilized by a density gradient has been applied to the small-scale fractionation of the proteins of the casein complex of cow's milk. 2. The alpha(s)- and beta-fractions from the milk of individual Ayrshire cows have been shown to behave as single homogeneous proteins on electrophoresis at two pH values in starch gels. 3. The alpha(s)-fraction has been found to be indistinguishable from alpha(s)-casein prepared by Ca(2+) fractionation of the same milk samples. 4. On the evidence of their electrophoretic behaviour in starch gels and their elementary analyses, alpha(1)- and alpha(s)-casein are concluded to be substantially the same protein.
摘要
- 利用密度梯度稳定柱中的区带电泳技术，对牛乳酪蛋白复合物中的蛋白质进行了小规模分级分离。2. 已证明，来自个体艾尔夏奶牛乳汁的α(s)-和β-组分在淀粉凝胶中两个pH值下进行电泳时表现为单一的均一蛋白质。3. 已发现α(s)-组分与通过对相同牛奶样品进行Ca(2+)分级分离制备的α(s)-酪蛋白无法区分。4. 根据它们在淀粉凝胶中的电泳行为及其元素分析的证据，得出α(1)-和α(s)-酪蛋白基本上是同一种蛋白质的结论。
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