Biedenbach M A, Kalu D N, Herbert D C
Department of Physiology, University of Texas Health Science Center, San Antonio 78284.
Mech Ageing Dev. 1992 Sep;65(2-3):111-25. doi: 10.1016/0047-6374(92)90028-c.
A quantitative morphometric study of the rat trigeminal ganglion was conducted to determine the changes that occur with aging. All measurements were tracked from young to old age in two rat groups simultaneously. One group was fed ad libitum, the other was maintained on restricted food intake from 6 weeks on. Immunocytochemical and radioimmunoassay techniques were used to study the neuron group that produces the peptide, CGRP and to compare it with the CGRP-negative neuron group. We observed that in the trigeminal ganglion, soma diameters and nucleus diameters of all neurons, whether CGRP positive or negative, increased modestly with age; so did total ganglion weight. Food restriction delayed, but did not prevent the increases in neuron diameters. No significant changes occurred as a function of age in the total number of neurons per ganglion, the ratio of CGRP positive to CGRP negative neurons and ganglion content of CGRP. Food restriction did not affect the parameters that remained constant with age. These findings are in contrast to the marked inhibitory effect of food restriction on age-related increase in thyroid calcitonin, a hormone that is encoded by the same gene as CGRP.
为了确定随着衰老而发生的变化，对大鼠三叉神经节进行了定量形态测量研究。在两个大鼠组中同时跟踪了从幼年到老年的所有测量值。一组自由进食，另一组从6周龄开始限制食物摄入量。使用免疫细胞化学和放射免疫分析技术研究产生肽CGRP的神经元群，并将其与CGRP阴性神经元群进行比较。我们观察到，在三叉神经节中，所有神经元（无论CGRP阳性还是阴性）的胞体直径和细胞核直径都随年龄适度增加；神经节总重量也是如此。食物限制延缓了，但并未阻止神经元直径的增加。每个神经节的神经元总数、CGRP阳性神经元与CGRP阴性神经元的比例以及神经节中CGRP的含量均未随年龄发生显著变化。食物限制并未影响随年龄保持恒定的参数。这些发现与食物限制对甲状腺降钙素（一种与CGRP由同一基因编码的激素）与年龄相关的增加的显著抑制作用形成对比。
