[Affinity modification of the 40S subparticle from human placenta with derivatives of pAUG and pAUGU3]. - Suppr | 超能文献

[Affinity modification of the 40S subparticle from human placenta with derivatives of pAUG and pAUGU3].

作者信息

Mundus D A, Malygin A A, Zenkova M A, Repkova M N, Ven'iaminova A G, Smailov S K, Li A V, Iskakov B K, Vratskikh L V, Iamkovoĭ V I

出版信息

Mol Biol (Mosk). 1992 Jul-Aug;26(4):949-56.

Abstract

Affinity labeling of 40S subunits from human placenta with 4-(N-2-chloroethyl-N-methylamino)benzylmethyl-[32P]phosphoamide s of oligoribonucleotides pAUG and pAUGU3 was studied. Covalent attachment of these derivatives to 40S subunits within the complexes with 40S subunits, formed in the presence of Met-tRNAf.eIF-2.GTP, was detected. Both rRNA and ribosomal proteins were modified. Fragments of 18S rRNA, containing sites of the reagent attachment were identified: 1058-1164 for pAUG derivative and 976-1057--for pAUG and pAUGU3 ones. The data obtained allowed to conclude that the presence of the neighbouring codon at the A-site, regardless of the presence of the tRNA in it, affects significantly the arrangement of the trinucleotide template in the codon-anticodon interaction region. The large subunit does not cause significant alterations in the structural organization of the codon-anticodon interaction region.

摘要

相似文献

1

[Affinity modification of the 40S subparticle from human placenta with derivatives of pAUG and pAUGU3].

Mol Biol (Mosk). 1992 Jul-Aug;26(4):949-56.

2

[Affinity modification of 40S ribosomal subparticles from human placenta by derivatives of oligoribonucleotides, containing an AUG codon. Ribosomal proteins, forming a codon-anticodon interaction region].

Mol Biol (Mosk). 1993 Jan-Feb;27(1):153-9.

3

[Affinity modification of 80S ribosomes from human placenta with mRNA analogs--derivatives of oligouridylates with alkylating groups at the 3'-end].

Mol Biol (Mosk). 1992 Jul-Aug;26(4):821-8.

4

[Affinity modification of 40S ribosomal subparticles from human placenta with mRNA analogs--AUGUnC oligoribonucleotide analogs with an alkylating group at the 3'-end].

Bioorg Khim. 1993 Mar;19(3):299-306.

5

[Affinity modification of 80S ribosomes from human placenta with mRNA analogs--derivatives of oligouridylates with and alkylating group at the 5'-end].

Bioorg Khim. 1993 Aug;19(8):791-9.

6

Structural arrangement of the codon-anticodon interaction area in human placenta ribosomes. Affinity labelling of the 40S subunits by derivatives of oligoribonucleotides containing the AUG codon.

Biochim Biophys Acta. 1993 Jun 25;1173(3):273-82. doi: 10.1016/0167-4781(93)90124-v.

7

Protein environment of mRNA at the decoding site of 80S ribosomes from human placenta as revealed from affinity labeling with mRNA analogs--derivatives of oligoribonucleotides.

Biochim Biophys Acta. 1997 Apr 10;1351(3):325-32. doi: 10.1016/s0167-4781(96)00224-2.

8

[Structural elements of 80S ribosomes located near the 5'-region of the mRNA-binding center].

Mol Biol (Mosk). 1994 Jul-Aug;28(4):918-25.

9

[Affinity modification of 80S ribosomes from human placenta by derivatives of tri- and hexauridylates as mRNA analogs].[以三尿苷酸和六尿苷酸衍生物作为mRNA类似物对人胎盘80S核糖体进行亲和修饰]

Mol Biol (Mosk). 1992 Mar-Apr;26(2):369-77.

10

Functional topography of human ribosomes as studied by affinity labeling with reactive mRNA analogs.

Biochimie. 1992 Apr;74(4):373-80. doi: 10.1016/0300-9084(92)90115-u.

文献AI研究员

20分钟写一篇综述，助力文献阅读效率提升50倍。

用中文搜PubMed

大模型驱动的PubMed中文搜索引擎

文档翻译

学术文献翻译模型，支持多种主流文档格式。