谷胱甘肽代谢在二甲基硫脲减轻阿霉素肾病蛋白尿中的作用

Role of glutathione metabolism in the reduction of proteinuria by dimethylthiourea in adriamycin nephrosis.

作者信息

Milner L S, Wei S H, Stohs S J, Eldeen Z M, Houser M T

机构信息

Department of Pediatrics, University of Nebraska Medical Center, Omaha.

出版信息

Nephron. 1992;62(2):192-7. doi: 10.1159/000187032.

DOI:10.1159/000187032

PMID:1436313

Abstract

Glutathione (GSH) metabolism, a tissue detoxification pathway, was evaluated in rats with adriamycin nephrosis (AN) treated with dimethylthiourea (DMTU), a free radical scavenger. After 7 days of DMTU, a significant reduction in proteinuria occurred as compared to AN controls (62.4 +/- 13.3 vs. 155.0 +/- 24.0 mg/24 h). A significant increase in renal cortical GSH content as well as glutathione peroxidase (GP) and transferase (GT) activities occurred in DMTU-treated rats as compared to controls. Glutathione monoethyl ester (GME) administration alone reduced proteinuria by 21% in AN, which was not significant despite a large increase in the renal GSH content, however, GP and GT activities were not increased by GME. We conclude that DMTU ameliorates glomerular injury in AN by stimulating GSH metabolism.

摘要

谷胱甘肽（GSH）代谢是一种组织解毒途径，本研究在接受自由基清除剂二甲基硫脲（DMTU）治疗的阿霉素肾病（AN）大鼠中对其进行了评估。给予DMTU 7天后，与AN对照组相比，蛋白尿显著减少（62.4±13.3 vs. 155.0±24.0 mg/24 h）。与对照组相比，DMTU治疗的大鼠肾皮质GSH含量以及谷胱甘肽过氧化物酶（GP）和转移酶（GT）活性显著增加。单独给予谷胱甘肽单乙酯（GME）可使AN大鼠蛋白尿减少21%，尽管肾GSH含量大幅增加，但差异不显著，而且GME并未增加GP和GT活性。我们得出结论，DMTU通过刺激GSH代谢改善AN中的肾小球损伤。