Manning S C, Wright C G
Department of Otolaryngology, University of Texas Southwestern, Medical Center, Dallas.
Otolaryngol Head Neck Surg. 1992 Nov;107(5):701-6. doi: 10.1177/019459989210700512.
After observing an association between vitamin A deficiency and otitis media among children in Micronesia, we sought to develop an animal model for vitamin A-deficiency-induced otitis media. Thirty juvenile guinea pigs were screened for absence for otitis and then divided into two groups. The experimental group was placed on a vitamin A-free diet, whereas control animals were fed a standard formula. The animals were followed by means of otologic examination and serum retinol determinations. When experimental animals demonstrated vitamin A deficiency, they were killed along with one control and temporal bones were harvested and prepared for histologic examination. None of the 15 controls demonstrated middle ear abnormalities. In the experimental group, 77% of temporal bones showed middle ear pathology consisting of either of subepithelial edema (27%) or frank otitis media (50%). These results indicate that the guinea pig is a suitable species for study of otitis media in association with vitamin A deficiency. We postulate that eustachian tube dysfunction is the primary mechanism for inducing middle ear inflammation in this animal model.
在观察到密克罗尼西亚儿童中维生素A缺乏与中耳炎之间存在关联后，我们试图建立一种维生素A缺乏诱导的中耳炎动物模型。对30只幼年豚鼠进行中耳炎筛查，然后将其分为两组。实验组采用无维生素A饮食，而对照动物则喂食标准配方饲料。通过耳科检查和血清视黄醇测定对动物进行跟踪观察。当实验动物出现维生素A缺乏时，将它们与一只对照动物一起处死，取出颞骨并准备进行组织学检查。15只对照动物均未出现中耳异常。在实验组中，77%的颞骨显示出中耳病理改变，包括上皮下水肿（27%）或明显的中耳炎（50%）。这些结果表明，豚鼠是研究与维生素A缺乏相关的中耳炎的合适物种。我们推测，咽鼓管功能障碍是该动物模型中诱导中耳炎症的主要机制。
