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E1a-responsive mammalian host/vector system for the stable high-level expression of secreted proteins.

作者信息

Berg D T, McClure D B, Grinnell B W

机构信息

Cardiovascular Department, Lilly Corporate Center, Indianapolis, IN 46285-0424.

出版信息

Nucleic Acids Res. 1992 Oct 25;20(20):5485-6. doi: 10.1093/nar/20.20.5485.

DOI:10.1093/nar/20.20.5485
PMID:1437573
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC334369/
Abstract
摘要

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本文引用的文献

1
Activation of the adenovirus and BK virus late promoters: effects of the BK virus enhancer and trans-acting viral early proteins.腺病毒和BK病毒晚期启动子的激活:BK病毒增强子及反式作用病毒早期蛋白的作用
Mol Cell Biol. 1986 Nov;6(11):3596-605. doi: 10.1128/mcb.6.11.3596-3605.1986.
2
E1A-induced enhancer activity of the poly(dG-dT).poly(dA-dC) element (GT element) and interactions with a GT-specific nuclear factor.E1A诱导的聚(dG-dT).聚(dA-dC)元件(GT元件)的增强子活性以及与GT特异性核因子的相互作用。
Mol Cell Biol. 1989 Nov;9(11):5248-53. doi: 10.1128/mcb.9.11.5248-5253.1989.
3
Signal and propeptide processing of human tissue plasminogen activator: activity of a pro-tPA derivative.人组织型纤溶酶原激活剂的信号肽及前肽加工:一种组织型纤溶酶原激活剂前体衍生物的活性
Biochem Biophys Res Commun. 1991 Sep 30;179(3):1289-96. doi: 10.1016/0006-291x(91)91713-m.
4
Characterization and novel purification of recombinant human protein C from three mammalian cell lines.从三种哺乳动物细胞系中对重组人蛋白C进行表征及新型纯化
Biotechnology (N Y). 1990 Jul;8(7):655-61. doi: 10.1038/nbt0790-655.
5
Nucleotide sequence analysis of the leader segments in a cloned copy of adenovirus 2 fiber mRNA.腺病毒2型纤维mRNA克隆拷贝中前导序列的核苷酸序列分析。
Cell. 1979 Apr;16(4):851-61. doi: 10.1016/0092-8674(79)90100-4.