Li K W, Smit A B, Geraerts W P
Faculty of Biology, Vrije Universiteit, Amsterdam, The Netherlands.
Peptides. 1992 Jul-Aug;13(4):633-8. doi: 10.1016/0196-9781(92)90166-z.
Mating as a male in the simultaneous hermaphrodite freshwater snail, Lymnaea stagnalis, comprises a series of complex behaviors that are a prelude to copulation. Copulatory behavior itself is assumed to be controlled by various types of peptidergic neurons as well as serotonergic cells. Here we report the primary structure of two peptides that were extracted from a cluster of neurons that innervates the penial complex and that is located in the anterior lobe of the right cerebral ganglion. The sequences of the peptides were determined as: Ala-Pro-Gly-Trp-amide and Ser-Gly-Ser-Asp-Tyr-Cys-Glu-Thr-Leu-Lys-Glu-Val-Ala-Asp-Glu-Tyr-Ile-Leu- Leu- Ser-Tyr-Lys-Ile-Glu-Glu-Gln-Arg-Ala-Ala-Asp-Cys-Gly-Gly-Glu-Pro-Pro-Asn- Ser- Gln(amide), respectively. The longer peptide is a homodimer. Both peptides are processed from the recently identified Ala-Pro-Gly-Trp-amide prohormone, which is expressed in the neurons of the anterior lobe of the right cerebral ganglion. Ala-Pro-Gly-Trp-amide could also be recovered from the penial complex. This peptide, when applied in vitro, inhibits the contractions of the penis retractor muscles evoked by serotonin in a dose-dependent fashion.
作为雌雄同体的淡水蜗牛——椎实螺进行雄性交配时，包括一系列复杂行为，这些行为是交配的前奏。交配行为本身被认为受多种肽能神经元以及5-羟色胺能细胞控制。在此，我们报告从一组支配阴茎复合体且位于右侧脑神经节前叶的神经元中提取的两种肽的一级结构。肽的序列分别确定为：丙氨酸-脯氨酸-甘氨酸-色氨酸-酰胺和丝氨酸-甘氨酸-丝氨酸-天冬氨酸-酪氨酸-半胱氨酸-谷氨酸-苏氨酸-亮氨酸-赖氨酸-谷氨酸-缬氨酸-丙氨酸-天冬氨酸-谷氨酸-酪氨酸-异亮氨酸-亮氨酸-丝氨酸-酪氨酸-赖氨酸-异亮氨酸-谷氨酸-谷氨酸-谷氨酰胺-精氨酸-丙氨酸-丙氨酸-天冬氨酸-半胱氨酸-甘氨酸-甘氨酸-谷氨酸-脯氨酸-脯氨酸-天冬酰胺-丝氨酸-谷氨酰胺（酰胺）。较长的肽是同二聚体。两种肽均由最近鉴定出的丙氨酸-脯氨酸-甘氨酸-色氨酸-酰胺前体激素加工而来，该前体激素在右侧脑神经节前叶的神经元中表达。丙氨酸-脯氨酸-甘氨酸-色氨酸-酰胺也可从阴茎复合体中回收。该肽在体外应用时，能以剂量依赖方式抑制5-羟色胺引起的阴茎收缩肌收缩。
