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Method for the determination of diphenhydramine in rabbit whole blood by high-performance liquid chromatography (HPLC) with ultraviolet (UV) detection in conjunction with gas chromatography (GC) with mass selective detection (MSD).

作者信息

Walters-Thompson K M, Mason W D

机构信息

Pharmacokinetics Laboratory, University of Missouri-Kansas City, School of Pharmacy 64108.

出版信息

Pharm Res. 1992 Jul;9(7):929-32. doi: 10.1023/a:1015861218342.

DOI:10.1023/a:1015861218342
PMID:1438008
Abstract

An HPLC/GC-MSD method for the determination of diphenhydramine in rabbit whole blood has been developed and validated. This method is based on a liquid-liquid extraction and reversed-phase chromatography with ultraviolet absorbance detection monitored at 258 nm. HPLC eluant fractions containing diphenhydramine and the internal standard, orphenadrine, were collected, reextracted, then subjected to GC-MSD analysis. Whole blood was utilized, thereby decreasing the required sample volume and increasing the sensitivity of the assay. Diphenhydramine concentrations can be quantitated over a range of 1 to 1000 ng/ml whole blood.

摘要

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本文引用的文献

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Determination of diphenhydramine in plasma by gas chromatography.气相色谱法测定血浆中的苯海拉明。
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Simultaneous identification and quantitation of diphenhydramine and methaqualone.
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