PC12细胞中习惯化的保留。
Retention of habituation in PC12 cells.
作者信息
Cheever L, Koshland D E
机构信息
Department of Molecular and Cell Biology, University of California, Berkeley 94720.
出版信息
Proc Natl Acad Sci U S A. 1992 Nov 1;89(21):10084-8. doi: 10.1073/pnas.89.21.10084.
The time course of habituation and recovery of neurotransmitter release was measured in neuronally differentiated PC12 cells stimulated with either acetylcholine or ATP. The release of norepinephrine in response to either stimulant declined exponentially with repeated presentation of that stimulant. When the stimulus was withheld, the cells' ability to respond recovered to initial levels with an exponential time course. The rate of response recovery depended on the stimulant used and, in the case of stimulation with acetylcholine, on the number of previous stimuli. After habituation and recovery, or partial recovery, of norepinephrine release, the response to a second series of repetitive stimuli declined more rapidly than in the naive case. This increase in habituation rate was dependent on the number of previous stimuli and, in the case of stimulation with acetylcholine, was stable with time for at least 90 min after stimulation. These phenomena are analogous to characteristics of short- and long-term memories of habituative learning observed in behavioral studies. Kinetic equations based on a putative reversible stimulation-dependent inactivation of the cellular response mechanism were used to analyze the rates of habituation and response recovery.
在用乙酰胆碱或ATP刺激的神经元分化PC12细胞中，测量了神经递质释放的习惯化和恢复的时间进程。对任何一种刺激物的反应中，去甲肾上腺素的释放随着该刺激物的重复呈现呈指数下降。当停止刺激时，细胞的反应能力以指数时间进程恢复到初始水平。反应恢复的速率取决于所使用的刺激物，对于乙酰胆碱刺激的情况，还取决于先前刺激的次数。在去甲肾上腺素释放的习惯化和恢复或部分恢复之后，对第二系列重复刺激的反应比未接触过刺激的情况下降得更快。这种习惯化速率的增加取决于先前刺激的次数，对于乙酰胆碱刺激的情况，在刺激后至少90分钟内随时间保持稳定。这些现象类似于行为研究中观察到的习惯化学习的短期和长期记忆的特征。基于假定的细胞反应机制的可逆刺激依赖性失活的动力学方程用于分析习惯化和反应恢复的速率。
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