Muschel R J, Zhang H B, Iliakis G, McKenna W G
Department of Pathology and Laboratory Medicine, University of Pennsylvania, Philadelphia.
Radiat Res. 1992 Nov;132(2):153-7.
The levels of cyclin B mRNA and protein rise rapidly in G2 + M phase, and fall at the end of mitosis. The studies described here were initiated to determine the effects of ionizing radiation on the level of cyclin B bearing in mind that the division delay induced by ionizing radiation might be influenced by the expression of cyclin B. After irradiation in S phase, the cyclin B mRNA in HeLa cells was measured as the cells proceeded through the cell cycle. Instead of the usual rise, after irradiation cyclin B mRNA levels remained low during the G2 delay. After irradiation in G2 phase, cyclin B mRNA was readily detectable although at slightly lower levels than in the controls. However, cyclin B protein was markedly decreased in amount.
细胞周期蛋白B的mRNA和蛋白质水平在G2+M期迅速升高，并在有丝分裂末期下降。鉴于电离辐射诱导的分裂延迟可能受细胞周期蛋白B表达的影响，本文所述研究旨在确定电离辐射对细胞周期蛋白B水平的影响。在S期进行辐射后，随着HeLa细胞进入细胞周期，对其细胞周期蛋白B的mRNA进行了测量。与正常升高情况不同，辐射后细胞周期蛋白B的mRNA水平在G2期延迟期间一直保持较低。在G2期进行辐射后，虽然细胞周期蛋白B的mRNA水平略低于对照组，但仍易于检测到。然而，细胞周期蛋白B的蛋白量明显减少。
