HICKMAN D D, FRENKEL A W
J Biophys Biochem Cytol. 1959 Oct;6(2):277-84. doi: 10.1083/jcb.6.2.277.
Cells from serial cultures of R. rubrum, grown anaerobically in the light, were harvested at intervals from (1/2) to 15 days and sectioned for electron microscopy by conventional methods. Cells of this species possess a multilayered outer envelope, and the external cell surface is differentiated into ridges extending parallel or obliquely to the long axis of the cell. Cells from very young cultures resemble non-photosynthetic bacteria and contain only a granular cytoplasm, scattered high-density particles, and low-density areas corresponding to the chromatin areas observed by light microscopy. They contain neither the chromatophores nor the lamellar systems assumed by previous investigators to be characteristic of this species when grown anaerobically in the light. Chromatophores appear in cells from cultures older than about 12 hours, while systems of paired lamellae appear along with the chromatophores in cells from cultures older than about 8 days. Divergent opinions concerning the occurrence of chromatophores or lamellae in this species can be resolved on the basis of the age of cultures used in previous studies. Other changes occurring in cells from cultures of increasing age include the appearance of granular and reticulate cytoplasmic bodies and vacuoles, extension of the chromatin areas, and the appearance of a single membrane enclosing several chromatophores.
深红螺菌在光照下厌氧培养的连续培养物中的细胞，每隔（1/2）至15天收获一次，并通过常规方法进行切片以用于电子显微镜观察。该物种的细胞具有多层外膜，细胞外表面分化为与细胞长轴平行或倾斜延伸的脊。来自非常年轻培养物的细胞类似于非光合细菌，仅含有颗粒状细胞质、分散的高密度颗粒以及对应于光学显微镜下观察到的染色质区域的低密度区域。它们既不含有载色体，也不含有先前研究者认为在光照下厌氧生长时该物种所特有的片层系统。载色体出现在培养约12小时以上的培养物的细胞中，而成对片层系统则与载色体一起出现在培养约8天以上的培养物的细胞中。关于该物种中载色体或片层出现情况的不同观点可以根据先前研究中所用培养物的年龄来解决。随着培养物年龄增加，细胞中发生的其他变化包括颗粒状和网状细胞质体及液泡的出现、染色质区域的扩展以及包围几个载色体的单层膜的出现。
