活化蛋白C对凝血酶原转化反应诱导的血小板聚集的抑制作用。

Inhibitory effect of activated protein C on platelet aggregation induced by the prothrombin-converting reaction.

作者信息

Komoriya K, Inoue K, Takahashi K, Suzuki K

机构信息

Teijin Institute for Bio-medical Research II, Tokyo, Japan.

出版信息

Thromb Res. 1992 Sep 15;67(6):705-10. doi: 10.1016/0049-3848(92)90074-k.

DOI:10.1016/0049-3848(92)90074-k

PMID:1440535

Abstract

The present study was undertaken to elucidate the effect on platelet aggregation of the prothrombin-converting reaction on platelets with or without activated protein C (APC). A reaction mixture of washed platelets from human individuals, Factor Xa and prothrombin markedly induced platelet aggregation; maximum aggregation rates, 31.3-92.5%, and times to reach to maximum aggregation, 11.6 to 20.1 min. This aggregation was inhibited by the addition of APC with 50% inhibition concentration (IC50) value of 14.4 U/ml. APC also inhibited thrombin generation in the reaction mixture in a dose-dependent manner with IC50 value of 0.96 U/ml. However, APC did not inhibit the thrombin (0.1 CU/ml)-induced platelet aggregation at concentrations of up to 30 U/ml. These findings suggest that APC has no direct inhibitory effect on platelet aggregation and that APC inhibits platelet aggregation through inhibition of thrombin generation.

摘要

本研究旨在阐明凝血酶原转化反应对有或没有活化蛋白C（APC）的血小板聚集的影响。来自人类个体的洗涤血小板、凝血因子Xa和凝血酶原的反应混合物显著诱导血小板聚集；最大聚集率为31.3 - 92.5%，达到最大聚集的时间为11.6至20.1分钟。加入APC可抑制这种聚集，50%抑制浓度（IC50）值为14.4 U/ml。APC还以剂量依赖方式抑制反应混合物中的凝血酶生成，IC50值为0.96 U/ml。然而，在浓度高达30 U/ml时，APC不抑制凝血酶（0.1 CU/ml）诱导的血小板聚集。这些发现表明，APC对血小板聚集没有直接抑制作用，并且APC通过抑制凝血酶生成来抑制血小板聚集。