水牛（水牛属）梭形肉孢子虫肉孢子囊不同组分中的一些葡萄糖代谢酶

Some glucose metabolic enzymes in various fractions of sarcocysts of Sarcocystis fusiformis of buffalo (Bubalus bubalis).

作者信息

Gupta R S, Kushwah H S, Kushwah A

机构信息

Department of Biochemistry, College of Veterinary Science and Animal Husbandry, M.P., India.

出版信息

Vet Parasitol. 1992 Sep;44(1-2):45-50. doi: 10.1016/0304-4017(92)90142-v.

DOI:10.1016/0304-4017(92)90142-v

PMID:1441191

Abstract

A comparative biochemical study on some enzymes of glycogenolysis, glycolysis and the hexose monophosphate shunt pathway in various fractions (cyst wall, cyst fluid and zoites) of the sarcocysts of Sarcocystis fusiformis from the oesophageal muscles of naturally infected Indian water buffalo (Bubalus bubalis) was carried out. The pattern and the magnitude of enzymic activity differed markedly in these fractions. Phosphorylase, hexokinase, aldolase and pyruvate kinase showed their highest levels of activity in the zoites fractions, whereas lactate dehydrogenase was the highest in cyst fluid. Alcohol dehydrogenases were non-detectable. Glucose 6-phosphate dehydrogenase and 6-phosphogluconate dehydrogenase were localized in the cyst wall only. Zoites were considered to be the most active metabolic sites for glucose breakdown.

摘要

对取自自然感染印度水牛（水牛属）食管肌肉中的梭形肉孢子虫肉孢子囊不同组分（囊壁、囊液和子孢子）中的糖原分解、糖酵解和磷酸己糖途径的某些酶进行了比较生化研究。这些组分中酶活性的模式和强度有显著差异。磷酸化酶、己糖激酶、醛缩酶和丙酮酸激酶在子孢子组分中显示出最高活性水平，而乳酸脱氢酶在囊液中活性最高。未检测到醇脱氢酶。6-磷酸葡萄糖脱氢酶和6-磷酸葡萄糖酸脱氢酶仅定位于囊壁。子孢子被认为是葡萄糖分解最活跃的代谢部位。