Qadota H, Ishii I, Fujiyama A, Ohya Y, Anraku Y
Department of Biology, Faculty of Science, University of Tokyo, Japan.
Yeast. 1992 Sep;8(9):735-41. doi: 10.1002/yea.320080906.
Two multicopy suppressors of the cal1-1 mutation in the yeast Saccharomyces cerevisiae have been isolated and characterized. They are identical to the yeast RHO1 and RHO2 genes, which encode putative small GTP-binding proteins. Multiple copies of either RHO gene suppressed temperature-sensitive growth of the cal1-1 mutant but did not suppress the cal1 null mutant. Genetic analysis suggests that overproduction of either RHO gene product acts for activation of the CAL1 gene product.
已分离并鉴定出酿酒酵母中cal1-1突变的两个多拷贝抑制子。它们与酵母RHO1和RHO2基因相同，这两个基因编码假定的小GTP结合蛋白。任一RHO基因的多个拷贝都抑制了cal1-1突变体的温度敏感生长，但不抑制cal1缺失突变体。遗传分析表明，任一RHO基因产物的过量表达都起到激活CAL1基因产物的作用。
