Nikolaeva A I, Nastichkin I A, Kabanova E A, Lapina E B, Krukovets I L
Zh Mikrobiol Epidemiol Immunobiol. 1992 Feb(2):15-8.
The comparative study of the lipopolysaccharides (LPS) of virulent and avirulent strains of S. sonnei, phase I (smooth colonies), has been made. Electrophoresis of LPS and subsequent densitometry of electrophoregrams have revealed the increase of the fraction of long 0-chains with a considerable number of recurring elements in 2 out of 3 LPS preparations obtained from avirulent shigellae. In mice immunized with these LPS preparations a considerably greater number of antibody-producing cells can be detected in Jerne's test on sheep red blood cells (SRBC) sensitized with the LPS of a virulent strain than on those sensitized with the above LPS preparations. Long 0-specific chains supposedly inhibit the fixation of individual complement components on the corresponding sensitized SRBC. The LPS of the third avirulent strain of S. sonnei, phase I, with transposon integrated into its genome, which has led to the formation of the avirulent variant of a previously virulent strain, seems to contain fine structural differences from the initial virulent strain. The immunogenicity of the LPS of this avirulent strain is greatly (3-4 times) decreased, which is manifested by the number of antibody-producing cells detected in Jerne's test on SRBC sensitized with LPS preparations obtained from these two strains.
对宋内志贺氏菌I相（光滑菌落）的强毒株和无毒株的脂多糖（LPS）进行了比较研究。对LPS进行电泳并随后对电泳图谱进行光密度测定，结果显示，从无毒志贺氏菌获得的3种LPS制剂中有2种制剂的长O链部分增加，其中有相当数量的重复元素。在用这些LPS制剂免疫的小鼠中，在用强毒株的LPS致敏的绵羊红细胞（SRBC）上进行的耶尔恩试验中检测到的产生抗体的细胞数量，比在用上述LPS制剂致敏的细胞上检测到的数量要多得多。长O特异性链可能会抑制单个补体成分在相应致敏SRBC上的固定。宋内志贺氏菌I相的第三种无毒株，其基因组中整合了转座子，这导致了先前强毒株的无毒变体的形成，其LPS似乎与初始强毒株在精细结构上存在差异。这种无毒株的LPS的免疫原性大大降低（3至4倍），这在用这两种菌株获得的LPS制剂致敏的SRBC上进行的耶尔恩试验中检测到的产生抗体的细胞数量上得到了体现。
