Han Y J, Zhang L, Cui R F
Department of Pharmacology, Tianjin Medical College.
Yao Xue Xue Bao. 1992;27(4):252-5.
Cinobufagin, one of the active principles extracted from toad venom, was studied on the isolated vas deferens of the guinea pig. The preparation was suspended in a bath containing 10 ml Krebs solution. Isometric tension of vas deferens was recorded on polygraph with a force displacement transducer. Cinobufagin caused a long lasting contraction of the vas deferens of the guinea pig at concentrations of 15-50 mumol/L. The contraction was inhibited following reserpinization and cold storage treatment. The cinobufagin induced contraction was partly blocked by pretreatment with phentolamine and verapamil. These results suggest that the cinobufagin induced contraction of vas deferens might be related to an action which promotes release of NA from the adrenergic nerve terminals.
华蟾酥毒基是从蟾蜍毒液中提取的活性成分之一，本研究以豚鼠离体输精管为实验对象展开。标本被悬浮于含有10毫升克雷布斯溶液的浴槽中。输精管的等长张力通过力位移换能器在多导生理记录仪上记录。华蟾酥毒基在浓度为15 - 50微摩尔/升时可引起豚鼠输精管的持久收缩。利血平化和冷藏处理后，该收缩作用受到抑制。酚妥拉明和维拉帕米预处理可部分阻断华蟾酥毒基诱导的收缩。这些结果表明，华蟾酥毒基诱导的输精管收缩可能与促进去甲肾上腺素能神经末梢释放去甲肾上腺素的作用有关。
