Schmidt M, Smith S D, Avery B, Purwantara B, Greve T
Department of Clinical Studies, Royal Veterinary and Agricultural University, Frederiksberg, Denmark.
Acta Vet Scand. 1992;33(3):237-43. doi: 10.1186/BF03547314.
Pregnancy rates after transfer of frozen/thawed bisected embryos, demiembryos, have until now been very low. In the present study it was attempted to improve the freezability rate of demiembryos by culturing them on a monolayer of bovine oviduct epithelial cells (BOEC) prior to freezing. The cultured frozen/thawed demiembryos showed a lower developmental rate than intact embryos. The pregnancy rate (23%) following transfer was not different from the pregnancy rate after transfer of unfrozen demiembryos (26%). The calving rate (4%), however, was significantly lower than the calving rate after transfer of fresh demiembryos (23%). Although the overall pregnancy rate achieved in this study was low, it can be concluded that a co-culture period upon BOEC prior to freezing does not improve the viability of frozen demiembryos.
迄今为止，冷冻/解冻后的二分胚胎（半胚）移植后的妊娠率一直很低。在本研究中，尝试通过在冷冻前将半胚培养在单层牛输卵管上皮细胞（BOEC）上来提高其冷冻存活率。培养后的冷冻/解冻半胚的发育率低于完整胚胎。移植后的妊娠率（23%）与未冷冻半胚移植后的妊娠率（26%）没有差异。然而，产犊率（4%）显著低于新鲜半胚移植后的产犊率（23%）。尽管本研究中实现的总体妊娠率较低，但可以得出结论，冷冻前在BOEC上进行共培养期并不能提高冷冻半胚的活力。
