The development of spontaneous motility in chick embryos interaction of picrotoxin and GABA.

The effect of the systemic administration of picrotoxin (1 mg/kg egg weight) and GABA (103 mg/kg e.w.) on the spontaneous motility of 11- to 21-day check embryos was studied intact eggs. Embryonal motility was recorded by the method of Kovach (1970). The activating effect of picrotoxin on 11- and 13-day embryos was unaffected by the administration of GABA. In 13- and 17-day-old embryos, GABA did not modify the paroxysmal effect of picrotoxin, but when the two substances were administered together, it prolonged the interparoxysmal intervals. The effect of GABA was most pronounced in 19- and 21-day-old embryos, in which it either stopped picrotoxin paroxysms from developing at all, or noticeably altered the length of the paroxysms, the amplitude of the movements and the length of the interparoxysmal intervals. The results show that the sensitivity of central apparatuses of embryonal spontaneous motility to GABA develops later than the early activatory effect of picrotoxin and are evidence of specific antagonism of these two neutrotropic substances.