Guttmann R D
Transplantation. 1977 Nov;24(5):316-24. doi: 10.1097/00007890-197711000-00002.
Utilizing a bone marrow cell immunization protocol designed to actively enhance renal or cardiac allografts, the in vitro mixed lymphocyte interaction has been studied. After immunization with allogeneic cells over a wide dose (10(5) to 10(8)) and time range, the mixed lymphocyte interaction using peripheral blood lymphocytes is reduced, and this reduction is shown in mixing experiments to be attributable to a circulating suppressor cell that has adherence characteristics of T lymphocytes. Specificity for in vivo generation of these circulating cells as well as specificity in the in vitro reaction was shown, although a nonspecific suppression background was observed. Allogeneic thymocytes, splenocytes, and platelets were effective in immunizing to generate these cells also. There was no general correlation with the ability to generate these cells and to actively enhance cardiac allografts, since suppression was seen after alloimmunization in strain combination that cannot be actively enhanced.
利用一种旨在积极增强肾或心脏同种异体移植的骨髓细胞免疫方案，对体外混合淋巴细胞相互作用进行了研究。在用不同剂量（10⁵至10⁸）和不同时间范围的同种异体细胞免疫后，使用外周血淋巴细胞的混合淋巴细胞相互作用降低，并且在混合实验中表明这种降低归因于具有T淋巴细胞黏附特性的循环抑制细胞。尽管观察到非特异性抑制背景，但显示了体内产生这些循环细胞的特异性以及体外反应的特异性。同种异体胸腺细胞、脾细胞和血小板在免疫以产生这些细胞方面也有效。产生这些细胞的能力与积极增强心脏同种异体移植的能力之间没有普遍相关性，因为在不能被积极增强的品系组合中，同种异体免疫后出现了抑制。
