Deterding L J, Dix K, Burka L T, Tomer K B
Laboratory of Molecular Biophysics, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina 27709.
Anal Chem. 1992 Nov 1;64(21):2636-41. doi: 10.1021/ac00045a029.
The capability of interfacing in vivo microdialysis with mass spectrometry has been demonstrated. The goal of this research was to demonstrate the feasibility of real-time analysis in biological systems using microdialysis in combination with tandem mass spectrometry (MS/MS). Microdialysis sampling was accomplished by surgically implanting a small microdialysis probe into a tissue or area of interest. Molecules diffuse through the membrane of the microdialysis probe due to concentration differences. These molecules are collected in a sample loop and analyzed by tandem mass spectrometry. Sequential injections can be made in as little as 2 min. This capability is advantageous in the study of molecules with very rapid elimination rates. Tris(2-chloroethyl)phosphate (TRCP) was used as a model compound in the development of this analytical technique. As an example of an application of the microdialysis/MS/MS technique, plasma concentration vs time curves were obtained and compared with the plasma concentration profiles obtained using conventional studies. For the microdialysis/MS/MS studies, the average slope from three animals was -0.086 min-1. In comparison, the average slope from four animals from the conventional studies was -0.035 min-1.
体内微透析与质谱联用的能力已得到证实。本研究的目的是证明使用微透析结合串联质谱（MS/MS）在生物系统中进行实时分析的可行性。微透析采样是通过手术将一个小型微透析探针植入感兴趣的组织或区域来完成的。由于浓度差异，分子会通过微透析探针的膜扩散。这些分子被收集在一个样品环中，并通过串联质谱进行分析。连续进样最短可在2分钟内完成。这种能力在研究消除速率非常快的分子时具有优势。磷酸三（2-氯乙基）酯（TRCP）被用作开发这种分析技术的模型化合物。作为微透析/MS/MS技术应用的一个例子，获得了血浆浓度随时间变化的曲线，并与使用传统研究获得的血浆浓度曲线进行了比较。对于微透析/MS/MS研究，三只动物的平均斜率为-0.086 min⁻¹。相比之下，传统研究中四只动物的平均斜率为-0.035 min⁻¹。
