氟烷可增强肺动脉内皮细胞类花生酸的释放。

Halothane enhances pulmonary artery endothelial eicosanoid release.

作者信息

Barnes S D, Martin L D, Wetzel R C

机构信息

Department of Anesthesiology/Critical Care Medicine, Johns Hopkins Medical Institutions, Baltimore, Maryland.

出版信息

Anesth Analg. 1992 Dec;75(6):1007-13. doi: 10.1213/00000539-199212000-00022.

DOI:10.1213/00000539-199212000-00022

PMID:1443680

Abstract

To determine whether anesthetics alter endothelial eicosanoid release, cultured bovine pulmonary artery endothelial cells were studied during constant flow and pressure perfusion at two oxygen tensions (hypoxia, 50 +/- 2 mm Hg; normoxia, 144 +/- 5 mm Hg; mean +/- SEM) with and without 1% halothane. Endothelialized microcarriers containing approximately 5 x 10(6) cells were loaded into cartridges and perfused (3 mL/min) with Krebs' solution (pH 7.4, at 37 degrees C) equilibrated with each gas mixture. Eicosanoids (6-keto prostaglandin F1 alpha, thromboxane B2, and total peptidoleukotrienes [C4, D4, E4, F4]) were measured by radioimmunoassay and quantified per gram of cellular protein per minute. Eicosanoid release did not vary over time. The 6-keto prostaglandin F1 alpha release increased during hypoxia (normoxia 291 +/- 27 vs hypoxia 395 +/- 35 ng.min-1 x g protein-1; P < 0.01). Halothane (H) increased release of each eicosanoid during both normoxia and hypoxia: 6-keto prostaglandin F1 alpha-normoxia 291 +/- 27 versus normoxia + H 356 +/- 32 ng.min-1 x g protein-1, hypoxia 395 +/- 35 versus hypoxia + H 464 +/- 40 ng.min-1 x g protein-1, P < 0.05; thromboxane B2-normoxia 19 +/- 2 versus normoxia + H26 +/- 2 ng.min-1 x g protein-1, hypoxia 20 +/- 2 versus hypoxia + H 38 +/- 5 ng.min-1 x g protein-1, P < 0.001; leukotriene-normoxia 363 +/- 35 versus normoxia + H 489 +/- 52 ng.min-1 x g protein-1, hypoxia 329 +/- 29 versus hypoxia + H 455 +/- 39 ng.min-1 x g protein-1, P = 0.001.(ABSTRACT TRUNCATED AT 250 WORDS)

摘要

为了确定麻醉剂是否会改变内皮细胞类花生酸的释放，我们在两种氧分压（低氧，50±2 mmHg；常氧，144±5 mmHg；平均值±标准误）下，对培养的牛肺动脉内皮细胞进行了恒流和压力灌注研究，灌注过程中分别添加和不添加1%氟烷。将含有约5×10⁶个细胞的内皮化微载体装入小柱，并用与每种气体混合物平衡的 Krebs 溶液（pH 7.4，37℃）以3 mL/分钟的速度进行灌注。通过放射免疫分析法测量类花生酸（6-酮前列腺素F1α、血栓素B2和总肽白三烯[C4、D4、E4、F4]），并按每分钟每克细胞蛋白进行定量。类花生酸的释放在不同时间没有变化。低氧时6-酮前列腺素F1α的释放增加（常氧291±27 vs 低氧395±35 ng·min⁻¹·g蛋白⁻¹；P<0.01）。氟烷（H）在常氧和低氧时均增加了每种类花生酸的释放：6-酮前列腺素F1α-常氧291±27 vs 常氧+H 356±32 ng·min⁻¹·g蛋白⁻¹，低氧395±35 vs 低氧+H 464±40 ng·min⁻¹·g蛋白⁻¹，P<0.05；血栓素B2-常氧19±2 vs 常氧+H 26±2 ng·min⁻¹·g蛋白⁻¹，低氧20±2 vs 低氧+H 38±5 ng·min⁻¹·g蛋白⁻¹，P<0.001；白三烯-常氧363±35 vs 常氧+H 489±52 ng·min⁻¹·g蛋白⁻¹，低氧329±29 vs 低氧+H 455±39 ng·min⁻¹·g蛋白⁻¹，P = 0.001。（摘要截断于250字）