Glass E J, Oliver R A, Williams J L, Millar P
AFRC Institute of Animal Physiology and Genetics Research, Edinburgh Research Station, Roslin, Midlothian, UK.
Anim Genet. 1992;23(2):97-111.
T-cell recognition of bovine MHC (BoLA) class II antigens was investigated in relation to BoLA class II polymorphisms defined by one-dimensional isoelectric focusing (1D-IEF). One-way mixed lymphocyte reactions (MLRs), and allospecific cell lines and clones were used. In general, T-cell responses correlated with the 1D-IEF defined haplotypes (EDF types). However, with MLRs some responses appeared to be associated with BoLA class I differences. All combinations of responder-stimulator pairs produced alloreactive T-cell responses both in MLR and in generation of allolines/clones. Thus allospecific lines and clones were generated to all EDF types tested. Splits in the IEF typing were observed with EDF6 and EDF3, indicating that distinct BoLA class II haplotypes are not necessarily distinguished by 1D-IEF alone. Furthermore, the patterns of reactivity with EDF3 expressing cells were complex with the T-cell specificities splitting EDF3 into several distinct types. Also, in some cases it was clear that more than one T-cell specificity per EDF type was detectable. Thus, allospecific lines and clones provide complementary and additional information to the 1D-IEF typing for polymorphism of the BoLA class II complex. This extra information is particularly important in terms of the functional significance of the BoLA complex for antigen presentation and immune response gene effects.
针对通过一维等电聚焦（1D-IEF）定义的牛主要组织相容性复合体（BoLA）II类抗原的多态性，研究了T细胞对其的识别。采用单向混合淋巴细胞反应（MLR）以及同种特异性细胞系和克隆进行研究。总体而言，T细胞反应与1D-IEF定义的单倍型（EDF类型）相关。然而，在MLR中，一些反应似乎与BoLA I类差异有关。在MLR以及生成同种细胞系/克隆的过程中，应答者 - 刺激者对的所有组合均产生了同种反应性T细胞反应。因此，针对所有测试的EDF类型均生成了同种特异性细胞系和克隆。在EDF6和EDF3中观察到IEF分型出现分裂，这表明仅通过1D-IEF不一定能区分不同的BoLA II类单倍型。此外，与表达EDF3的细胞的反应模式很复杂，T细胞特异性将EDF3分为几种不同类型。而且，在某些情况下很明显，每种EDF类型可检测到不止一种T细胞特异性。因此，同种特异性细胞系和克隆为BoLA II类复合体多态性的1D-IEF分型提供了补充和额外信息。就BoLA复合体对抗原呈递和免疫反应基因效应的功能意义而言，这些额外信息尤为重要。
