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齿垢密螺旋体天冬氨酸氨甲酰基转移酶基因的克隆与表达

Cloning and expression of the aspartate carbamoyltransferase gene from Treponema denticola.

作者信息

Ishihara K, Ishihara M, Takazoe I, Okuda K

机构信息

Department of Microbiology, Tokyo Dental College, Chiba, Japan.

出版信息

Appl Environ Microbiol. 1992 Oct;58(10):3399-403. doi: 10.1128/aem.58.10.3399-3403.1992.

DOI:10.1128/aem.58.10.3399-3403.1992
PMID:1444372
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC183110/
Abstract

Treponema denticola seems to play a central role in the etiology of human periodontal disease. We have cloned an antigenic protein-coding sequence from T. denticola ATCC 33520. The protein-coding region was found to be a 3-kbp HindIII-HindIII fragment. The open reading frame consists of 1,426 bp and codes for a protein with an M(r) of 54,919. The deduced amino acid sequence showed 33.8% homology with that of the aspartate carbamoyltransferase of Escherichia coli. The gene products showed aspartate carbamoyltransferase activity.

摘要

齿垢密螺旋体似乎在人类牙周病的病因学中起核心作用。我们从齿垢密螺旋体ATCC 33520中克隆了一个抗原蛋白编码序列。发现该蛋白编码区是一个3-kbp的HindIII - HindIII片段。开放阅读框由1426 bp组成，编码一个分子量为54919的蛋白质。推导的氨基酸序列与大肠杆菌天冬氨酸氨甲酰基转移酶的氨基酸序列具有33.8%的同源性。该基因产物表现出天冬氨酸氨甲酰基转移酶活性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e536/183110/940c07b50f7e/aem00051-0201-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e536/183110/72cbf96ffe56/aem00051-0200-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e536/183110/940c07b50f7e/aem00051-0201-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e536/183110/72cbf96ffe56/aem00051-0200-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e536/183110/940c07b50f7e/aem00051-0201-a.jpg

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本文引用的文献

1
ELECTRON MICROSCOPIC OBSERVATIONS ON THE BACTERIAL FLORA OF ACUTE NECROTIZING ULCERATIVE GINGIVITIS.急性坏死性溃疡性龈炎细菌区系的电子显微镜观察
J Periodontol (1930). 1965 Jul-Aug;36:328-39. doi: 10.1902/jop.1965.36.4.328.
2
METABOLISM OF LEPTOSPIRAE. I. UTILIZATION OF AMINO ACIDS AND PURINE, AND PYRIMIDINE BASES.钩端螺旋体的代谢。一、氨基酸、嘌呤及嘧啶碱的利用
Arch Biochem Biophys. 1964 Sep;107:459-70. doi: 10.1016/0003-9861(64)90302-9.
3
5-FLUOROURACIL AS A SELECTIVE AGENT FOR GROWTH OF LEPTOSPIRAE.5-氟尿嘧啶作为钩端螺旋体生长的选择剂
来自嗜热真细菌海栖热袍菌的天冬氨酸转氨甲酰酶：融合的催化和调节多肽形成一种别构酶。
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4
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Infect Immun. 1996 Dec;64(12):5178-86. doi: 10.1128/iai.64.12.5178-5186.1996.
5
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J Bacteriol. 1996 May;178(9):2489-97. doi: 10.1128/jb.178.9.2489-2497.1996.
6
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Infect Immun. 1995 Apr;63(4):1147-52. doi: 10.1128/iai.63.4.1147-1152.1995.
J Bacteriol. 1964 Feb;87(2):422-6. doi: 10.1128/jb.87.2.422-426.1964.
4
Relationship between the percentage of subgingival spirochetes and the severity of periodontal disease.龈下螺旋体百分比与牙周病严重程度之间的关系。
J Periodontol. 1982 Sep;53(9):550-6. doi: 10.1902/jop.1982.53.9.550.
5
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Suppression of human lymphocyte responses by oral spirochetes: a monocyte-dependent phenomenon.口腔螺旋体对人淋巴细胞反应的抑制作用：一种单核细胞依赖现象。
J Immunol. 1984 Apr;132(4):2039-45.
7
Evolutionary divergence of genes for ornithine and aspartate carbamoyl-transferases--complete sequence and mode of regulation of the Escherichia coli argF gene; comparison of argF with argI and pyrB.鸟氨酸和天冬氨酸氨甲酰基转移酶基因的进化分歧——大肠杆菌argF基因的完整序列及调控模式；argF与argI和pyrB的比较
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8
The DNA sequence of argI from Escherichia coli K12.来自大肠杆菌K12的精氨酸酶I的DNA序列。
Nucleic Acids Res. 1983 Dec 10;11(23):8509-18. doi: 10.1093/nar/11.23.8509.
9
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Proc Natl Acad Sci U S A. 1984 Jan;81(1):115-9. doi: 10.1073/pnas.81.1.115.
10
New M13 vectors for cloning.用于克隆的新型M13载体。
Methods Enzymol. 1983;101:20-78. doi: 10.1016/0076-6879(83)01005-8.