Piette J P, Idziak E S
Department of Microbiology, McGill University, Sainte Anne de Bellevue, Québec, Canada.
Appl Environ Microbiol. 1992 Sep;58(9):2783-91. doi: 10.1128/aem.58.9.2783-2791.1992.
A study was undertaken to investigate the factors involved in the adhesion of Pseudomonas fluorescens to model meat surfaces (tendon slices). Adhesion was fast (less than 2.5 min) and was not suppressed by killing the cells with UV, gamma rays, or heat, indicating that physiological activity was not required. In various salt solutions (NaCl, KCl, CaCl2, MgCl2), adhesion increased with increasing ionic strength up to 10 to 100 mM, suggesting that, at low ionic strengths, electrostatic interactions were involved in the adhesion process. At higher ionic strengths (greater than 10 to 100 mM) or in the presence of Al3+ ions, adhesion was sharply reduced. Selectively blocking of carboxyl or amino groups at the cell surface by chemical means did not affect adhesion. These groups are therefore not directly involved in an adhesive bond with tendon. Given a sufficient cell concentration (10(10) CFU.ml-1) in the adhesion medium, the surface of tendon was almost entirely covered with adherent bacteria. This suggests that if the adhesion is specific, the attachment sites on the tendon surface must be located within collagen or proteoglycan molecules.
开展了一项研究，以调查荧光假单胞菌附着于模拟肉类表面（肌腱切片）的相关因素。附着过程迅速（少于2.5分钟），且用紫外线、γ射线或加热杀死细胞并不能抑制附着，这表明不需要生理活性。在各种盐溶液（氯化钠、氯化钾、氯化钙、氯化镁）中，离子强度增加至10至100 mM时附着增加，这表明在低离子强度下，静电相互作用参与了附着过程。在较高离子强度（大于10至100 mM）或存在铝离子的情况下，附着急剧减少。通过化学方法选择性阻断细胞表面的羧基或氨基并不影响附着。因此，这些基团并不直接参与与肌腱的黏附键。在附着培养基中若有足够的细胞浓度（10¹⁰ CFU.ml⁻¹），肌腱表面几乎完全被附着细菌覆盖。这表明，如果附着具有特异性，肌腱表面的附着位点必定位于胶原蛋白或蛋白聚糖分子内。
