Shimizu A
Am J Vet Res. 1977 Sep;38(9):1389-92.
Lysogenicity in chicken coagulase-positive staphylococci was tested by incubating the strains in the presence of mitomycin C. Of 88 strains tested, 84 (95.5%) were proved to be phage carriers and 81 were susceptible to any of the phages. The lysogenic strains were detected with almost equal frequency from both of typeable and untypeable strains by the international phages. Sixteen phages (CH phages) were isolated from chicken lysogenic strains, and their usefulness for the typing of chicken staphylococci was evaluated. Of 122 strains examined, 101 (82.8%) were found to be typeable with the CH phages at a routine test dilution (RTD). About 82% of strains untypeable by the phages of the international series were lysed by one or more of the CH phages. The phages seemed to be highly specific to chicken staphylococci, because they lysed only a few strains of animal origin other than chicken. Thus, the 16 phages newly established were found to have significant advantages in typing chicken strains.
通过在丝裂霉素C存在的情况下培养菌株，对鸡凝固酶阳性葡萄球菌的溶原性进行了检测。在检测的88株菌株中，有84株（95.5%）被证明是噬菌体携带者，81株对任何一种噬菌体敏感。通过国际噬菌体从可分型和不可分型菌株中检测到溶原性菌株的频率几乎相等。从鸡溶原性菌株中分离出16种噬菌体（CH噬菌体），并评估了它们对鸡葡萄球菌分型的实用性。在检测的122株菌株中，有101株（82.8%）在常规试验稀释度（RTD）下能用CH噬菌体分型。约82%不能被国际系列噬菌体分型的菌株能被一种或多种CH噬菌体裂解。这些噬菌体似乎对鸡葡萄球菌具有高度特异性，因为它们只裂解了少数除鸡以外的动物源菌株。因此，新建立的16种噬菌体在鸡菌株分型中具有显著优势。
