Nathan E, Huang H F, Pogach L, Giglio W, Bogden J D, Seebode J
Section of Urology, University of Medicine and Dentistry of New Jersey, Newark.
Arch Environ Health. 1992 Sep-Oct;47(5):370-5. doi: 10.1080/00039896.1992.9938377.
This study was conducted to determine the effects of lead on Sertoli cell function. Androgen binding protein and inhibin in testicular fluids and classical parameters of the hypothalamic-pituitary-gonadal axis were measured in adult male rats. For 10 wk, the rats were given water that contained 0.05%, 0.1%, 0.5%, and 1% lead acetate. Serum follicle-stimulating hormone, luteinizing hormone, and testosterone levels in all animals that ingested lead were normal at the middle and end of the experiment, as was the pituitary content of follicle-stimulating hormone and luteinizing hormone. Histologic examination revealed no disruption of spermatogenesis. Distribution of androgen binding protein in serum, seminiferous tubular fluid, and interstitial fluid was normal, as was the concentration of inhibin in interstitial fluid and seminiferous tubular fluid. However, a significant increase in epididymal androgen binding protein level and a decrease in seminal vesicle weight were observed in rats that ingested water containing 1% lead acetate. These results suggest that the effect of lead on spermatogenesis is not marked in adult Sprague Dawley rats, nor does Sertoli cell function appear to be affected adversely. Lead has been reported to alter in vitro metabolic function of Sertoli cells obtained from 16- to 21-d-old Sprague Dawley rats, and the Sertoli cells of juvenile animals may be more susceptible to lead than those of adult animals. The significant decrease in seminal vesicle weight and the abnormal epididymal androgen binding protein content indicate that lead could affect the male reproductive function in Sprague Dawley rats via its action on male accessory organs.
本研究旨在确定铅对支持细胞功能的影响。对成年雄性大鼠的睾丸液中的雄激素结合蛋白和抑制素以及下丘脑 - 垂体 - 性腺轴的经典参数进行了测量。在10周内，给大鼠饮用含有0.05%、0.1%、0.5%和1%醋酸铅的水。在实验中期和末期，所有摄入铅的动物的血清促卵泡激素、促黄体生成素和睾酮水平均正常，垂体中促卵泡激素和促黄体生成素的含量也正常。组织学检查显示精子发生未受破坏。血清、生精小管液和间质液中雄激素结合蛋白的分布正常，间质液和生精小管液中抑制素的浓度也正常。然而，在饮用含1%醋酸铅水的大鼠中，观察到附睾雄激素结合蛋白水平显著升高，精囊重量下降。这些结果表明，铅对成年斯普拉格 - 道利大鼠的精子发生影响不明显，支持细胞功能似乎也未受到不利影响。据报道，铅会改变从出生16至21天的斯普拉格 - 道利大鼠获得的支持细胞的体外代谢功能，幼年动物的支持细胞可能比成年动物的支持细胞对铅更敏感。精囊重量的显著下降和附睾雄激素结合蛋白含量异常表明，铅可能通过对雄性附属器官的作用影响斯普拉格 - 道利大鼠的雄性生殖功能。
