Koiwa M, Shiga H, Nakamura H, Yoshino S
Department of Rheumatology, Dai-ichi Hospital, Nippon Medical School.
Arerugi. 1992 Sep;41(9):1423-9.
The presence of beta-endorphin (beta-end) was immunohistologically identified in synovial tissue samples biopsied from patients with rheumatoid arthritis (RA) and osteoarthritis (OA). The amount of beta-end in culture supernatants of synovial tissue explants was also determined by RIA. beta-end was strongly detected in mainly superficial synovial cells, vascular endothelial cells and a few synovial interstitial cells in RA patients, but not in OA patients. In RA patients the beta-end concentration was significantly higher in the supernatants of tissue explants (26.4 +/- 8.3 pg/ml) than in the plasma of the same patients (15.3 +/- 2.5 pg/ml) (p < 0.01). Using isolated synovial cells, the beta-end concentration in the culture supernatants of non-adherent cells (19.4 pg/ml) was higher than that of adherent cells (4.0 pg/ml). It is suggested that beta-end is produced by non-adherent cells such as lymphocytes and neutrophils in addition to synovial lining cells and endothelial cells and may play some role in the pathology of RA synovial inflammation.
在类风湿性关节炎（RA）和骨关节炎（OA）患者的滑膜组织活检样本中，通过免疫组织化学方法鉴定出了β-内啡肽（β-end）的存在。还通过放射免疫分析法（RIA）测定了滑膜组织外植体培养上清液中β-end的含量。在RA患者中，主要在滑膜浅层细胞、血管内皮细胞和少数滑膜间质细胞中强烈检测到β-end，而在OA患者中则未检测到。在RA患者中，组织外植体上清液中的β-end浓度（26.4±8.3 pg/ml）显著高于同一患者血浆中的浓度（15.3±2.5 pg/ml）（p<0.01）。使用分离的滑膜细胞，非贴壁细胞培养上清液中的β-end浓度（19.4 pg/ml）高于贴壁细胞（4.0 pg/ml）。提示β-end除了由滑膜衬里细胞和内皮细胞产生外，还由淋巴细胞和中性粒细胞等非贴壁细胞产生，可能在RA滑膜炎症的病理过程中发挥一定作用。
